Inhibitory function of p21Cip1/WAF1 in differentiation of primary mouse keratinocytes independent of cell cycle control.

Détails

ID Serval
serval:BIB_484316395499
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inhibitory function of p21Cip1/WAF1 in differentiation of primary mouse keratinocytes independent of cell cycle control.
Périodique
Science
Auteur(s)
Di Cunto F., Topley G., Calautti E., Hsiao J., Ong L., Seth P.K., Dotto G.P.
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
1998
Volume
280
Numéro
5366
Pages
1069-1072
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The cyclin-dependent kinase inhibitor p21(Cip1/WAF1) has been implicated as an inducer of differentiation. However, although expression of p21 is increased in postmitotic cells immediately adjacent to the proliferative compartment, its expression is decreased in cells further along the differentiation program. Expression of the p21 protein was decreased in terminally differentiated primary keratinocytes of mice, and this occurred by a proteasome-dependent pathway. Forced expression of p21 in these cells inhibited the expression of markers of terminal differentiation at both the protein and messenger RNA levels. These inhibitory effects on differentiation were not observed with a carboxyl-terminal truncation mutant or with the unrelated cyclin-dependent kinase inhibitor p16(INK4a), although all these molecules exerted similar inhibition of cell growth. These findings reveal an inhibitory role of p21 in the late stages of differentiation that does not result from the effects of p21 on the cell cycle.
Mots-clé
Acetylcysteine/analogs & derivatives, Acetylcysteine/pharmacology, Adenoviridae/genetics, Adenoviridae/physiology, Animals, Animals, Newborn, Cell Cycle, Cell Differentiation, Cells, Cultured, Cyclin-Dependent Kinase Inhibitor p21, Cyclin-Dependent Kinases/antagonists & inhibitors, Cyclin-Dependent Kinases/metabolism, Cyclins/genetics, Cyclins/metabolism, Enzyme Inhibitors/metabolism, Gene Expression Regulation, Keratinocytes/cytology, Keratinocytes/metabolism, Leupeptins/pharmacology, Membrane Proteins/biosynthesis, Membrane Proteins/genetics, Mice, Mutation, Promoter Regions, Genetic, Protein Precursors/biosynthesis, Protein Precursors/genetics, RNA, Messenger/genetics, RNA, Messenger/metabolism, Succinates/pharmacology, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 14:59
Dernière modification de la notice
20/08/2019 13:55
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