Methods for human CD8(+) T lymphocyte proteome analysis.

Détails

ID Serval
serval:BIB_482C6A985EF0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Methods for human CD8(+) T lymphocyte proteome analysis.
Périodique
Methods In Molecular Biology
Auteur⸱e⸱s
Thadikkaran L., Rufer N., Benay C., Crettaz D., Tissot J.D.
ISSN
1064-3745[print], 1064-3745[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
484
Pages
45-65
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
Mots-clé
Blotting, Western/methods, CD8-Positive T-Lymphocytes/chemistry, CD8-Positive T-Lymphocytes/cytology, Cell Culture Techniques, Cell Fractionation/instrumentation, Cell Fractionation/methods, Cell Nucleus/chemistry, Electrophoresis, Gel, Two-Dimensional/methods, Humans, Mass Spectrometry/methods, Molecular Sequence Data, Proteome/analysis
Pubmed
Web of science
Création de la notice
30/07/2008 9:46
Dernière modification de la notice
20/08/2019 13:54
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