Expression of interleukin-3 and tumor necrosis factor-beta mRNAs in cultured microglia.

Détails

ID Serval
serval:BIB_477B15CDDB5F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of interleukin-3 and tumor necrosis factor-beta mRNAs in cultured microglia.
Périodique
Journal of Neuroimmunology
Auteur⸱e⸱s
Appel K., Honegger P., Gebicke-Haerter P.J.
ISSN
0165-5728 (Print)
ISSN-L
0165-5728
Statut éditorial
Publié
Date de publication
1995
Volume
60
Numéro
1-2
Pages
83-91
Langue
anglais
Résumé
The function of interleukin-3 (or multi-CSF) in the hemopoietic system has been studied in great detail. Although its growth promoting activity on brain microglial cells has been confirmed both in vitro and in vivo, its presence in the brain and even in cultured brain cells has repeatedly been questioned. We have shown recently that isolated rat microglia express mRNA(IL-3) and synthesize IL-3 polypeptide. It is shown here by use of the PCR method, that mRNA(IL-3) is found also in C6 glioblastoma, in rat aggregate cultures, and in newborn and adult rat brain. Quantitation of amplified cDNA(IL-3) was achieved by non-competitive RT-PCR using an elongated internal standard. IL-3 messenger RNA was almost undetectable in vivo and low in (serum-free) aggregate cultures. In isolated microglia, mRNA(IL-3) was increased upon treatment with LPS, PHA, with the cytokines IL-1 or TNF-alpha, with retinoic acid, dbcAMP or the phorbol ester TPA. Effects of LPS were inhibited by dexamethasone, while the glucocorticoid by itself had no effect on basal IL-3 expression. LPS increased mRNA(IL-3) in a concentration-dependent manner beginning with 10 pg/ml and reaching plateau levels at 10 ng/ml. LPS also increased mRNAs of TNF-alpha and TNF-beta. TNF-alpha mRNA was already detectable in untreated microglia and LPS-increased levels were sustained for a few days. In contrast, TNF-beta mRNA was observed only between 4 and 16 h of LPS incubation. It was absent in LPS-free microglia, and after 24 h of LPS-treatment or later.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Animals, Base Sequence, Brain/cytology, Brain/metabolism, Cells, Cultured, Gene Expression, Interleukin-3/genetics, Lipopolysaccharides/pharmacology, Lymphotoxin-alpha/genetics, Microglia/metabolism, Molecular Probes, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger/metabolism, Rats, Rats, Wistar, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 13:11
Dernière modification de la notice
20/08/2019 13:53
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