Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor.
Détails
ID Serval
serval:BIB_45B26CE86496
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor.
Périodique
Micron
ISSN
1878-4291 (Electronic)
ISSN-L
0968-4328
Statut éditorial
Publié
Date de publication
01/2017
Peer-reviewed
Oui
Volume
92
Pages
19-24
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
The mouse serotonin 5-HT <sub>3A</sub> receptor is a homo-pentameric ligand-gated ion channel (pLGIC) mediating fast excitatory neurotransmission in the central nervous system. The molecular mechanism of ion permeation of 5-HT <sub>3A</sub> receptors triggered by the neurotransmitter serotonin is not yet fully understood. The recent X-ray structure of the mouse serotonin 5-HT <sub>3A</sub> receptor in complex with a stabilizing nanobody revealed for the first time the entire structure of a mammalian pLGIC in detergent. Structural information of the receptor in a lipid bilayer however is still limited primarily due to the lack of 2D crystals of the receptor in a lipid bilayer. Here we present our results on the formation and improvement of diffracting 2D crystals of the mouse 5-HT <sub>3A</sub> by limited proteolysis and addition of conformational nanobodies.
Mots-clé
Animals, Cryoelectron Microscopy, Crystallization, Crystallography, Imaging, Three-Dimensional, Mice, Models, Molecular, Molecular Conformation, Receptors, Serotonin, 5-HT3/chemistry, Receptors, Serotonin, 5-HT3/ultrastructure, Single-Domain Antibodies, 2D crystals, 5-HT3 receptor, Electron crystallography, Nanobody, Pentameric ligand-gated ion channel
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/06/2023 15:02
Dernière modification de la notice
20/07/2023 5:57
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