Lipid metabolite involvement in the activation of the human heme oxygenase-1 gene.

Détails

ID Serval
serval:BIB_4538
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lipid metabolite involvement in the activation of the human heme oxygenase-1 gene.
Périodique
Free Radical Biology and Medicine
Auteur(s)
Basu-Modak S., Lüscher P., Tyrrell R.M.
ISSN
0891-5849
Statut éditorial
Publié
Date de publication
1996
Volume
20
Numéro
7
Pages
887-897
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Cellular effects of ultraviolet A (UVA) radiation include peroxidation of membrane lipids as well as a decrease in intracellular glutathione. We have investigated whether damage to membrane lipids is involved in the activation of the human heme oxygenase-1 gene by UVA. Irradiation of human skin fibroblasts in the presence of the lipophilic antioxidants, butylated hydroxytoluene and alpha-tocopherol, enhances the UVA-induced HO-1 mRNA accumulation, suggesting that peroxidation of plasma membrane lipids is not involved. Furthermore, sodium ascorbate, which induces lipid peroxidation mainly in the plasma membrane, induces HO-1 mRNA to low levels only. The decrease in GSH by UVA radiation is not affected by the presence of the lipophilic antioxidants while ascorbate treatment increases the intracellular GSH by twofold above controls. These results indicate that peroxidation of internal membrane lipids, a decrease in the intracellular GSH levels and the integrity of the plasma membrane are all important for the UVA-induction of heme oxygenase-1. Both nonenzymatic as well as enzymatic lipid peroxidation metabolites are inducers of heme oxygenase-1. The nonenzymatic lipid peroxidation product 4-hydroxynonenal induces heme oxygenase-1 mRNA up to 40-fold and the phospholipase metabolites diacylglycerol and arachidonic acid induce this mRNA by three-to sixfold above basal levels. We also demonstrate that the cyclooxygenase metabolites of arachidonic acid are important for the UVA-activation of the heme oxygenase-1 gene.
Mots-clé
Aldehydes/metabolism, Arachidonic Acid/metabolism, Cell Line, Cyclic AMP/physiology, Diglycerides/metabolism, Free Radicals, Gene Expression Regulation, Enzymologic/physiology, Heme Oxygenase (Decyclizing)/genetics, Humans, Lipid Peroxidation/physiology, Membrane Lipids/metabolism, Membrane Lipids/radiation effects, Oxidation-Reduction, Phospholipase D/metabolism, RNA, Messenger/metabolism, Type C Phospholipases/metabolism, Ultraviolet Rays
Pubmed
Web of science
Création de la notice
19/11/2007 12:40
Dernière modification de la notice
20/08/2019 13:49
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