Tyrosinase, the key enzyme in melanin synthesis, is expressed in murine brain.

Détails

ID Serval
serval:BIB_4535
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tyrosinase, the key enzyme in melanin synthesis, is expressed in murine brain.
Périodique
European Journal of Biochemistry / Febs
Auteur(s)
Tief K., Hahne M., Schmidt A., Beermann F.
ISSN
0014-2956 (Print)
ISSN-L
0014-2956
Statut éditorial
Publié
Date de publication
1996
Volume
241
Numéro
1
Pages
12-16
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Tyrosinase is one of the key enzymes in mammalian melanin synthesis. The pigment is produced in two different cell types: the pigmented epithelial cell of the retina, and the melanocyte, a cell of neural-crest origin. We recently showed that a fusion gene between regulatory sequences of tyrosinase gene (tyr) and the beta-galactosidase gene (lacZ), when introduced into transgenic mice, resulted in embryonic expression in presumptive pigment cells but also in cells populations along the entire neural tube. This expression in the developing brain was striking, and we therefore asked whether this would still be detectable after birth. Transgenic mice carrying the tyr-lacZ fusion gene showed beta-galactosidase expression in adult brain. On Western blots, we detected tyrosinase-specific bands of 65-68 kDa in brain and eye. Using an affinity-purified antibody, we showed that detection of tyrosinase is specific and competed off by the presence of the cognate tyrosinase-derived peptide. However, neither tyrosine hydroxylase nor Dopa oxidase activity were detected in protein extracts of brain. We therefore suggest that tyrosinase is present in brain but either not functional or catalyzing different reactions compared to pigment cells.
Mots-clé
Animals, Blotting, Northern, Blotting, Western, Brain/enzymology, Cell Extracts/chemistry, Gene Expression Regulation/genetics, Lac Operon/genetics, Melanins/biosynthesis, Melanocytes/metabolism, Mice, Mice, Transgenic, Monophenol Monooxygenase/analysis, Monophenol Monooxygenase/metabolism, Polymerase Chain Reaction, RNA, Messenger/analysis, RNA, Messenger/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Sodium Channels, Tyrosine 3-Monooxygenase/analysis, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 12:40
Dernière modification de la notice
20/08/2019 13:49
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