Human herpesvirus 8 immunostaining: a sensitive and specific method for diagnosing Kaposi sarcoma in paraffin-embedded sections

Détails

ID Serval
serval:BIB_44D90822B42E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human herpesvirus 8 immunostaining: a sensitive and specific method for diagnosing Kaposi sarcoma in paraffin-embedded sections
Périodique
American Journal of Clinical Pathology
Auteur(s)
Robin  Y. M., Guillou  L., Michels  J. J., Coindre  J. M.
ISSN
0002-9173 (Print)
Statut éditorial
Publié
Date de publication
2004
Volume
121
Numéro
3
Pages
330-334
Notes
PT - Journal Article
Résumé
Human herpesvirus 8 (HHV-8) is recognized as a major causal agent of Kaposi sarcoma (KS), and it has been detected in all epidemiologic variants of KS. Until now, detection of HHV-8 in paraffin-embedded sections was done mostly by using reverse transcriptase-polymerase chain reaction. To assess the sensitivity and specificity of an anti-HHV-8 antibody and its potential usefulness for separating KS from its mimickers, we immunostained 72 KS samples and 108 samples of potential mimickers of KS with the monoclonal antibody latent nuclear antigen-1 (LNA-1; Advanced Biotechnologies, Columbia, MD). Cases of KS included all epidemiologic variants of the disease. Non-KS lesions included 34 angiosarcomas, 4 kaposiform hemangioendotheliomas, and 70 various benign vascular lesions. Immunostaining for CD31, CD34, and/or von Willebrand factor (factor VIII) also were performed in all cases. All but 1 case of KS (sensitivity, 99%) and none of the non-KS lesions (specificity, 100%) stained with the LNA-1 anti-HHV-8 antibody. The LNA-1 anti-HHV-8 antibody is a reliable marker of all variants of KS. Because KS mimickers are consistently negative for this marker, its use for diagnostic purposes is recommended
Mots-clé
Adult/Aged/Aged,80 and over/Antibodies,Monoclonal/diagnostic use/Female/Herpesvirus 8,Human/isolation & purification/Humans/Immunohistochemistry/Male/Middle Aged/Nuclear Proteins/biosynthesis/immunology/Phosphoproteins/Sarcoma,Kaposi/diagnosis/virology/Sensitivity and Specificity/Skin Diseases/Tumor Virus Infections
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 18:36
Dernière modification de la notice
20/08/2019 13:49
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