Selective binding of specific mouse genomic DNA fragments by mouse vimentin filaments in vitro.

Détails

ID Serval
serval:BIB_44C2513558A0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Selective binding of specific mouse genomic DNA fragments by mouse vimentin filaments in vitro.
Périodique
DNA and cell biology
Auteur(s)
Wang X., Tolstonog G., Shoeman R.L., Traub P.
ISSN
1044-5498 (Print)
ISSN-L
1044-5498
Statut éditorial
Publié
Date de publication
03/1996
Peer-reviewed
Oui
Volume
15
Numéro
3
Pages
209-225
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Mouse vimentin intermediate filaments (IFs) reconstituted in vitro were analyzed for their capacity to select certain DNA sequences from a mixture of about 500-bp-long fragments of total mouse genomic DNA. The fragments preferentially bound by the IFs and enriched by several cycles of affinity binding and polymerase chain reaction (PCR) amplification were cloned and sequenced. In general, they were G-rich and highly repetitive in that they often contained Gn, (GT)n, and (GA)n repeat elements. Other, more complex repeat sequences were identified as well. Apart from the capacity to adopt a Z-DNA and triple helix configuration under superhelical tension, many fragments were potentially able to form cruciform structures and contained consensus binding sites for various transcription factors. All of these sequence elements are known to occur in introns and 5'/3'-flanking regions of genes and to play roles in DNA transcription, recombination and replication. A FASTA search of the EMBL data bank indeed revealed that sequences homologous to the mouse repetitive DNA fragments are commonly associated with gene-regulatory elements. Unexpectedly, vimentin IFs also bound a large number of apparently overlapping, AT-rich DNA fragments that could be aligned into a composite sequence highly homologous to the 234-bp consensus centromere repeat sequence of gamma-satellite DNA. Previous experiments have shown a high affinity of vimentin for G-rich, repetitive telomere DNA sequences, superhelical DNA, and core histones. Taken together, these data support the hypothesis that, after penetration of the double nuclear membrane via an as yet unidentified mechanism, vimentin IFs cooperatively fix repetitive DNA sequence elements in a differentiation-specific manner in the nuclear periphery subjacent to the nuclear lamina and thus participate in the organization of chromatin and in the control of transcription, replication, and recombination processes. This includes aspects of global regulation of gene expression such as the position effects associated with translocation of genes to heterochromatic centromere and telomere regions of the chromosomes.
Mots-clé
Animals, Base Sequence, Binding Sites, DNA/metabolism, DNA-Binding Proteins/metabolism, Intermediate Filaments/metabolism, Mice, Molecular Sequence Data, Nucleic Acid Conformation, Protein Binding, Sequence Alignment, Sequence Homology, Nucleic Acid, Transcription Factors/metabolism, Vimentin/metabolism
Pubmed
Web of science
Création de la notice
15/12/2017 16:28
Dernière modification de la notice
14/01/2020 6:26
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