Iodide excess regulates its own efflux: a possible involvement of pendrin.

Détails

ID Serval
serval:BIB_44976378D3D3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Iodide excess regulates its own efflux: a possible involvement of pendrin.
Périodique
American journal of physiology. Cell physiology
Auteur(s)
Calil-Silveira J., Serrano-Nascimento C., Kopp P.A. (co-dernier), Nunes M.T.
ISSN
1522-1563 (Electronic)
ISSN-L
0363-6143
Statut éditorial
Publié
Date de publication
01/04/2016
Peer-reviewed
Oui
Volume
310
Numéro
7
Pages
C576-82
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Adequate iodide supply and metabolism are essential for thyroid hormones synthesis. In thyrocytes, iodide uptake is mediated by the sodium-iodide symporter, but several proteins appear to be involved in iodide efflux. Previous studies demonstrated that pendrin is able to mediate apical efflux of iodide in thyrocytes. Acute iodide excess transiently impairs thyroid hormone synthesis, a phenomenon known as the Wolff-Chaikoff effect. Although the escape from this inhibitory effect is not completely understood, it has been related to the inhibition of sodium-iodide symporter-mediated iodide uptake. However, the effects of iodide excess on iodide efflux have not been characterized. Herein, we investigated the consequences of iodide excess on pendrin abundance, subcellular localization, and iodide efflux in rat thyroid PCCl3 cells. Our results indicate that iodide excess increases pendrin abundance and plasma membrane insertion after 24 h of treatment. Moreover, iodide excess increases pendrin half-life. Finally, iodide exposure also increases iodide efflux from PCCl3 cells. In conclusion, these data suggest that pendrin may have an important role in mediating iodide efflux in thyrocytes, especially under conditions of iodide excess.
Mots-clé
Animals, Blotting, Western, Cell Line, Chloride-Bicarbonate Antiporters/metabolism, Flow Cytometry, Fluorescent Antibody Technique, Rats, Real-Time Polymerase Chain Reaction, Sodium Iodide/metabolism, Sodium Iodide/pharmacology, Sulfate Transporters, Thymocytes/drug effects, Thymocytes/metabolism, Wolff-Chaikoff effect escape, iodide efflux, iodide excess, pendrin
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/12/2020 14:59
Dernière modification de la notice
28/12/2020 7:26
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