A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast.

Détails

ID Serval
serval:BIB_447A9B7631D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast.
Périodique
EMBO Journal
Auteur(s)
Perrod S., Cockell M.M., Laroche T., Renauld H., Ducrest A.L., Bonnard C., Gasser S.M.
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
01/2001
Peer-reviewed
Oui
Volume
20
Numéro
1-2
Pages
197-209
Langue
anglais
Notes
Publication types: Journal Article
Résumé
In budding yeast, the silent information regulator Sir2p is a nuclear NAD-dependent deacetylase that is essential for both telomeric and rDNA silencing. All eukaryotic species examined to date have multiple homologues of Sir two (HSTs), which share a highly conserved globular core domain. Here we report that yeast Hst2p and a mammalian Hst2p homologue, hSirT2p, are cytoplasmic in yeast and human cells, in contrast to yHst1p and ySir2p which are exclusively nuclear. Although yHst2p cannot restore silencing in a sir2 deletion, overexpression of yHst2p influences nuclear silencing events in a SIR2 strain, derepressing subtelomeric silencing while increasing repression in the rDNA. In contrast, a form of ySir2p carrying a point mutation in the conserved core domain disrupts both telomeric position effect (TPE) and rDNA repression at low expression levels. This argues that non-nuclear yHst2p can compete for a substrate or ligand specifically required for telomeric, and not rDNA repression.
Mots-clé
Amidohydrolases/chemistry, Amidohydrolases/genetics, Amino Acid Substitution, Cell Nucleus/genetics, Cytosol/enzymology, DNA, Fungal/genetics, DNA, Ribosomal/genetics, Gene Silencing, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Phylogeny, Recombinant Proteins/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Sirtuins, Telomere/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/03/2008 10:15
Dernière modification de la notice
20/08/2019 14:48
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