Neurofilament triplet proteins are restricted to a subset of neurons in the rat neocortex.

Détails

ID Serval
serval:BIB_44287DE4A40B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Neurofilament triplet proteins are restricted to a subset of neurons in the rat neocortex.
Périodique
Journal of Chemical Neuroanatomy
Auteur⸱e⸱s
Kirkcaldie M.T., Dickson T.C., King C.E., Grasby D., Riederer B.M., Vickers J.C.
ISSN
0891-0618 (Print)
ISSN-L
0891-0618
Statut éditorial
Publié
Date de publication
2002
Volume
24
Numéro
3
Pages
163-171
Langue
anglais
Résumé
The cellular localisation of neurofilament triplet subunits was investigated in the rat neocortex. A subset of mainly pyramidal neurons showed colocalisation of subunit immunolabelling throughout the neocortex, including labelling with the antibody SMI32, which has been used extensively in other studies of the primate cortex as a selective cellular marker. Neurofilament-labelled neurons were principally localised to two or three cell layers in most cortical regions, but dramatically reduced labelling was present in areas such as the perirhinal cortex, anterior cingulate and a strip of cortex extending from caudal motor regions through the medial parietal region to secondary visual areas. However, quantitative analysis demonstrated a similar proportion (10-20%) of cells with neurofilament triplet labelling in regions of high or low labelling. Combining retrograde tracing with immunolabelling showed that cellular content of the neurofilament proteins was not correlated with the length of projection. Double labelling immunohistochemistry demonstrated that neurofilament content in axons was closely associated with myelination. Analysis of SMI32 labelling in development indicated that content of this epitope within cell bodies was associated with relatively late maturation, between postnatal days 14 and 21. This study is further evidence of a cell type-specific regulation of neurofilament proteins within neocortical neurons. Neurofilament triplet content may be more closely related to the degree of myelination, rather than the absolute length, of the projecting axon.
Mots-clé
Animals, Neocortex/chemistry, Neocortex/cytology, Neurofilament Proteins/analysis, Neurons/chemistry, Neurons/cytology, Rats, Rats, Wistar
Pubmed
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
20/08/2019 14:48
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