Cell kinetic studies of chick brain cells in culture: an autoradiographic study with [3H]- and [14C]-thymidine.

Détails

ID Serval
serval:BIB_43E84E19F6A7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell kinetic studies of chick brain cells in culture: an autoradiographic study with [3H]- and [14C]-thymidine.
Périodique
Cell and tissue kinetics
Auteur(s)
Barakat I., Korr H., Schultze-Maurer B., Sensenbrenner M.
ISSN
0008-8730
Statut éditorial
Publié
Date de publication
1985
Volume
18
Numéro
1
Pages
45-57
Langue
anglais
Résumé
Labelling index, S-phase duration and cell-cycle time of proliferating brain cells from 6-day-old chick embryos in culture were investigated autoradiographically after labelling with [3H]- and/or [14C]-thymidine. The dissociated cells were cultured in the absence or in the presence of brain extract from 8-day-old chick embryos. Cultures contained essentially two cell types, which could be easily distinguished by the size of their nuclei: small nuclei identified as belonging to precursor cells of neurons and large nuclei corresponding to astroglial cells. The labelling index of astroglial cells (16.4%) was about 2 times higher than that of the neuronal cells (9.9%). Under the influence of brain extract the labelling index of neuroblasts was nearly doubled while that of the astroglial cells remained nearly unchanged. From double-labelling experiments with [3H]- and [14C]-thymidine, the same S-phase duration of about 7 hr was found for both cell types cultured with or without brain extract. A cell-cycle duration of 39 hr for neuronal and of 29 hr for astroglial cells was found. The cycle times remained constant under the influence of brain extract. From the measured data mentioned above, a growth fraction of 50% (neuroblasts) and 68% (astroglial cells) was calculated in control cultures without brain extract. After addition of brain extract, the growth fraction increased for both cell types (neuroblasts: 92%; astroglial cells: 80%). The results demonstrate that more cells proliferate in the presence of brain extract, but the durations of the S-phase and the cell cycle remain unchanged.
Mots-clé
Animals, Autoradiography, Brain, Carbon Radioisotopes, Cell Cycle, Cells, Cultured, Chick Embryo, Thymidine, Tritium
Pubmed
Web of science
Création de la notice
30/03/2009 10:28
Dernière modification de la notice
20/08/2019 14:48
Données d'usage