Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy.
Détails
Télécharger: 37298500_BIB_43DC8DC3137A.pdf (2970.55 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_43DC8DC3137A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy.
Périodique
International journal of molecular sciences
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Statut éditorial
Publié
Date de publication
31/05/2023
Peer-reviewed
Oui
Volume
24
Numéro
11
Pages
9550
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
All coronaviruses are characterized by spike glycoproteins whose S1 subunits contain the receptor binding domain (RBD). The RBD anchors the virus to the host cellular membrane to regulate the virus transmissibility and infectious process. Although the protein/receptor interaction mainly depends on the spike's conformation, particularly on its S1 unit, their secondary structures are poorly known. In this paper, the S1 conformation was investigated for MERS-CoV, SARS-CoV, and SARS-CoV-2 at serological pH by measuring their Amide I infrared absorption bands. The SARS-CoV-2 S1 secondary structure revealed a strong difference compared to those of MERS-CoV and SARS-CoV, with a significant presence of extended β-sheets. Furthermore, the conformation of the SARS-CoV-2 S1 showed a significant change by moving from serological pH to mild acidic and alkaline pH conditions. Both results suggest the capability of infrared spectroscopy to follow the secondary structure adaptation of the SARS-CoV-2 S1 to different environments.
Mots-clé
Humans, Middle East Respiratory Syndrome Coronavirus, SARS-CoV-2/metabolism, Spike Glycoprotein, Coronavirus/metabolism, COVID-19, Spectrum Analysis, ATR-IR spectroscopy, MERS-CoV, SARS-CoV, SARS-CoV-2, conformation, secondary structure, spike glycoproteins
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/06/2023 8:39
Dernière modification de la notice
08/08/2024 6:32