Efficiency of Several Cytochrome P450 Biomarkers in Highlighting the Exposure of Daphnia magna to an Organophosphate Pesticide.
Détails
Télécharger: toxics-10-00482.pdf (2376.63 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_4388D0508E9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Efficiency of Several Cytochrome P450 Biomarkers in Highlighting the Exposure of Daphnia magna to an Organophosphate Pesticide.
Périodique
Toxics
ISSN
2305-6304 (Electronic)
ISSN-L
2305-6304
Statut éditorial
Publié
Date de publication
17/08/2022
Peer-reviewed
Oui
Volume
10
Numéro
8
Pages
482
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
The cytochromes P450 (CYP450) represent a major enzyme family operating mostly in the first step of xenobiotic detoxification in aquatic organisms. The ability to measure these CYP450 enzymes' activities provides a crucial tool to understand organisms' response to chemical stressors. However, research on CYP450 activity measurement is still limited and has had variable success. In the present study, we optimize, compile, and compare existing scientific information and techniques for a series of CYP450 biomarkers (EROD, MROD, ECOD, APND, and ERND) used on Daphnia magna. Additionally, we explored these CYP450 biomarkers' activities through the first 5 days of life of daphnids, providing a link between their age and sensitivity to chemicals. In the experiment, daphnids were exposed to an organophosphate pesticide (diazinon) from birth to measure the molecular response of the detoxification process. Our results suggest EROD as the most applicable biomarker for organisms such as D. magna, with a higher organophosphate detoxification rate in daphnids that are 2 and 5 days old. Additionally, a larger body size allowed a more accurate EROD measurement; hence, we emphasize the use of 5-day-old daphnids when analyzing their detoxification response.
Mots-clé
Chemical Health and Safety, Health, Toxicology and Mutagenesis, Toxicology, Daphnia magna, biomarker, cytochrome P450, xenobiotic detoxification
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/08/2022 15:36
Dernière modification de la notice
10/07/2024 7:10