Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland).

Détails

ID Serval
serval:BIB_437194A7AE18
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland).
Périodique
Chemosphere
Auteur⸱e⸱s
Thévenon F., Adatte T., Wildi W., Poté J.
ISSN-L
0045-6535
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
86
Pages
468-476
Langue
anglais
Résumé
This study investigates faecal indicator bacteria (FIB), multiple antibiotic resistant (MAR), and antibiotic resistance genes (ARGs), of sediment profiles from different parts of Lake Geneva (Switzerland) over the last decades. MARs consist to expose culturable Escherichia coli (EC) and Enterococcus (ENT) to mixed five antibiotics including Ampicillin, Tetracycline, Amoxicillin, Chloramphenicol and Erythromycin. Culture-independent is performed to assess the distribution of ARGs responsible for, β-lactams (blaTEM; Amoxicillin/Ampicillin), Streptomycin/Spectinomycin (aadA), Tetracycline (tet) Chloramphenicol (cmlA) and Vancomycin (van). Bacterial cultures reveal that in the sediments deposited following eutrophication of Lake Geneva in the 1970s, the percentage of MARs to five antibiotics varied from 0.12% to 4.6% and 0.016% to 11.6% of total culturable EC and ENT, respectively. In these organic-rich bacteria-contaminated sediments, the blaTEM resistant of FIB varied from 22% to 48% and 16% to 37% for EC and ENT respectively, whereas the positive PCR assays responsible for tested ARGs were observed for EC, ENT, and total DNA from all samples. The aadA resistance gene was amplified for all the sediment samples, including those not influenced by WWTP effluent water. Our results demonstrate that bacteria MARs and ARGs highly increased in the sediments contaminated with WWTP effluent following the cultural eutrophication of Lake Geneva. Hence, the human-induced changing limnological conditions highly enhanced the sediment microbial activity, and therein the spreading of antibiotic resistant bacteria and genes in this aquatic environment used to supply drinking water in a highly populated area. Furthermore, the presence of the antibiotic resistance gene aadA in all the studied samples points out a regional dissemination of this emerging contaminant in freshwater sediments since at least the late nineteenth century.
Mots-clé
Drug Resistance, Bacterial/genetics, Enterococcus/genetics, Environmental Monitoring, Escherichia coli/genetics, Eutrophication, Genes, Bacterial, Geologic Sediments/chemistry, Geologic Sediments/microbiology, Lakes/chemistry, Lakes/microbiology, Microbial Sensitivity Tests, Type="Geographic">Switzerland, Water Pollution/statistics & numerical data
Création de la notice
24/03/2012 10:41
Dernière modification de la notice
20/08/2019 13:47
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