Novel Chlamydiales strains isolated from a water treatment plant.

Détails

ID Serval
serval:BIB_436E82E769F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Novel Chlamydiales strains isolated from a water treatment plant.
Périodique
Environmental Microbiology
Auteur⸱e⸱s
Corsaro D., Feroldi V., Saucedo G., Ribas F., Loret J.F., Greub G.
ISSN
1462-2920[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
11
Numéro
1
Pages
188-200
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Chlamydiae are obligate intracellular bacteria infecting free-living amoebae, vertebrates and some invertebrates. Novel members are regularly discovered, and there is accumulating evidence supporting a very important diversity of chlamydiae in the environment. In this study, we investigated the presence of chlamydiae in a drinking water treatment plant. Samples were used to inoculate Acanthamoeba monolayers (Acanthamoeba co-culture), and to recover autochthonous amoebae onto non-nutritive agar. Chlamydiae were searched for by a pan-chlamydia 16S rRNA gene PCR from both Acanthamoeba co-cultures and autochthonous amoebae, and phylotypes determined by 16S rRNA gene sequencing. Autochthonous amoebae also were identified by 18S rRNA gene amplification and sequencing. From a total of 79 samples, we recovered eight chlamydial strains by Acanthamoeba co-culture, but only one of 28 amoebae harboured a chlamydia. Sequencing results and phylogenetic analysis showed our strains belonging to four distinct chlamydial lineages. Four strains, including the strain recovered within its natural host, belonged to the Parachlamydiaceae; two closely related strains belonged to the Criblamydiaceae; two distinct strains clustered with Rhabdochlamydia spp.; one strain clustered only with uncultured environmental clones. Our results confirmed the usefulness of amoeba co-culture to recover novel chlamydial strains from complex samples and demonstrated the huge diversity of chlamydiae in the environment, by identifying several new species including one representing the first strain of a new family.
Mots-clé
Amoeba/genetics, Amoeba/microbiology, Animals, Chlamydiales/classification, Chlamydiales/genetics, Cluster Analysis, Coculture Techniques, DNA, Bacterial/chemistry, DNA, Bacterial/genetics, DNA, Protozoan/chemistry, DNA, Protozoan/genetics, DNA, Ribosomal/chemistry, DNA, Ribosomal/genetics, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Protozoan/genetics, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 18S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Water Microbiology
Pubmed
Web of science
Création de la notice
12/10/2009 14:57
Dernière modification de la notice
20/08/2019 14:47
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