ET-1 and NOS III gene expression regulation by plaque-free and plaque-prone hemodynamic conditions
Détails
ID Serval
serval:BIB_4303A4D417A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
ET-1 and NOS III gene expression regulation by plaque-free and plaque-prone hemodynamic conditions
Périodique
Biorheology
ISSN
0006-355X (Print)
Statut éditorial
Publié
Date de publication
2003
Volume
40
Numéro
1-3
Pages
289-97
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Résumé
Both plaque-free and plaque-prone hemodynamic environments induce an increase in the oxidative state of endothelial cells (ECs), whereas differential gene expression regulation was described in cells exposed to these conditions. In order to investigate the role of the increased oxidative state in flow-regulation of gene expression, we first exposed EC to non-pulsed unidirectional shear stress. These conditions only slightly increases ECs oxidative state and endothelin-1 (ET-1) mRNA expression, whereas endothelial nitric oxide synthase (NOS III) mRNA level were significantly up-regulated. On the contrary, both ET-1 and NOS III gene expression were significantly induced in EC exposed to pulsed-unidirectional flow (plaque-free). Only ET-1 gene expression was up-regulated by oscillatory flow (plaque-prone). Moreover, use of an antioxidant only partially inhibited NOS III gene up-regulation by unidirectional flow, whereas it completely abrogated ET-1 gene up-regulation by unidirectional and oscillatory flows. Thus suggesting that mechanical forces regulate gene expression in ECs both via oxidative stress-dependent and -independent mechanisms.
Mots-clé
Animals
Antioxidants/pharmacology
Arteriosclerosis/genetics/*metabolism/physiopathology
Cattle
Cell Culture Techniques/methods
Endothelin-1/*biosynthesis/genetics
Endothelium, Vascular/*metabolism
Gene Expression Regulation/drug effects/*physiology
Hemodynamic Processes
Hemorheology
Mechanotransduction, Cellular/physiology
Nitric Oxide Synthase/*biosynthesis/genetics
Oxidation-Reduction
Pulsatile Flow/physiology
RNA, Messenger/genetics
Stress, Mechanical
Pubmed
Web of science
Création de la notice
17/01/2008 16:38
Dernière modification de la notice
20/08/2019 13:46