Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity

Détails

ID Serval
serval:BIB_42AA8C5E75D4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity
Périodique
Journal of Clinical Investigation
Auteur⸱e⸱s
Jongeneel  C. V., Quackenbush  E. J., Ronco  P., Verroust  P., Carrel  S., Letarte  M.
ISSN
0021-9738 (Print)
Statut éditorial
Publié
Date de publication
02/1989
Volume
83
Numéro
2
Pages
713-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
We have previously reported that the amino acid sequence of the common acute lymphoblastic leukemia antigen (CALLA, CD10) translated from a normal human kidney cDNA clone is identical to that of neutral endopeptidase (NEP, EC 3.4.24.11). In this study, we show that by flow cytometry, a monoclonal antibody (135A3) produced against rabbit NEP reacted selectively with leukemia and melanoma cell lines expressing CALLA on their surface. A glycoprotein of apparent Mr 100,000 was immunoprecipitated from surface labeled NALM-1 leukemia or Mel-1477 melanoma cells with monoclonal antibodies to NEP (135A3) or CALLA (44C10). mRNAs hybridizing to a NEP-specific probe were present in CALLA+ leukemia and melanoma cell lines, but absent from CALLA- lines. NEP enzymatic activity was detected on intact cells from CALLA+ lines, but not CALLA- lines. The activity was blocked by two selective inhibitors of NEP, thiorphan and phosphoramidon. CALLA antigen purified from the NALM-6 leukemic cell line by affinity to 44C10-IgG Sepharose retained a peptidase activity that was completely blocked by thiorphan and phosphoramidon. Thus the CALLA antigen present at the surface of leukemia and melanoma cell lines is an enzymatically active neutral endopeptidase.
Mots-clé
Antibodies, Monoclonal Antigens, Differentiation/analysis Antigens, Neoplasm/analysis/*biosynthesis Cell Line Flow Cytometry Humans Leukemia, Lymphocytic, Acute/*immunology Melanoma/*metabolism Molecular Weight Neprilysin/*metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:39
Dernière modification de la notice
20/08/2019 13:45
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