Essential role of the glycosyltransferase sxc/Ogt in polycomb repression.

Détails

ID Serval
serval:BIB_408AF6B7F87E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Essential role of the glycosyltransferase sxc/Ogt in polycomb repression.
Périodique
Science
Auteur(s)
Gambetta M.C., Oktaba K., Müller J.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
03/07/2009
Peer-reviewed
Oui
Volume
325
Numéro
5936
Pages
93-96
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Polycomb group proteins are conserved transcriptional repressors that control animal and plant development. Here, we found that the Drosophila Polycomb group gene super sex combs (sxc) encodes Ogt, the highly conserved glycosyltransferase that catalyzes the addition of N-acetylglucosamine (GlcNAc) to proteins in animals and plants. Genome-wide profiling in Drosophila revealed that GlcNAc-modified proteins are highly enriched at Polycomb response elements. Among different Polycomb group proteins, Polyhomeotic is glycosylated by Sxc/Ogt in vivo. sxc/Ogt-null mutants lacked O-linked GlcNAcylation and failed to maintain Polycomb transcriptional repression even though Polycomb group protein complexes were bound at their target sites. Polycomb repression appears to be a critical function of Sxc/Ogt in Drosophila and may be mediated by the glycosylation of Polyhomeotic.
Mots-clé
Acetylglucosamine/metabolism, Animals, Chromatin/metabolism, Chromatin Immunoprecipitation, DNA-Binding Proteins/metabolism, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, Gene Silencing, Genes, Insect, Glycosylation, Nucleoproteins/metabolism, Polycomb Repressive Complex 1, Polycomb-Group Proteins, Protein Binding, Protein Subunits/metabolism, Repressor Proteins/chemistry, Repressor Proteins/genetics, Repressor Proteins/metabolism, Transcription, Genetic
Pubmed
Web of science
Création de la notice
19/07/2018 10:56
Dernière modification de la notice
21/08/2019 6:35
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