Multicenter evaluation study on a new HEp2 ANA screening enzyme immune assay

Détails

ID Serval
serval:BIB_4027D03DC611
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Multicenter evaluation study on a new HEp2 ANA screening enzyme immune assay
Périodique
Journal of Autoimmunity
Auteur(s)
Bayer  P. M., Bauerfeind  S., Bienvenu  J., Fabien  N., Frei  P. C., Gilburd  B., Heide  K. G., Hoier-Madsen  M., Meroni  P. L., Monier  J. C., Monneret  G., Panzeri  P., Shoenfeld  Y., Spertini  F., Wiik  A.
ISSN
0896-8411 (Print)
Statut éditorial
Publié
Date de publication
08/1999
Volume
13
Numéro
1
Pages
89-93
Notes
Comparative Study
Journal Article
Multicenter Study --- Old month value: Aug
Résumé
The COBAS Core HEp2 ANA enzyme immune assay (EIA) was evaluated in a precision and a clinical sample study in comparison to indirect immunofluorescence assay (IFA) on HEp2-cells. In the precision study the COBAS Core EIA yielded intraassay coefficient variations (CVs) mostly below 9%, and interassay CVs between 4.7% and 10.4%. When comparing the COBAS Core EIA to IFA, the results corresponded well in healthy subjects, systemic lupus erythematosus, mixed connective tissue disease and rheumatoid arthritis. In the case of Sjogren's syndrome and scleroderma patients the COBAS Core EIA yielded a lower rate of positive results compared to IFA. This discrepancy may be explained by the lack of detection of autoantibodies to nuclear antigens that can be identified only by IFA due to their compartmentalization and higher localized antigen density in HEp2 cells. The discrepancies in the group of dermato/polymyositis patients are due to the fact that the EIA contains mainly nuclear antigens and was able to detect only antibodies against the cytoplasmic Jo1 antigen that was added to the HEp2 nuclear extract. Routine sera were also evaluated; good agreement was found in sera from patients attending tertiary reference centres for autoimmune diseases but a higher number of discrepancies was reported in sera from unselected populations.
Mots-clé
Adult Antibodies, Antinuclear/*analysis/blood Arthritis, Rheumatoid/immunology Cell Line Dermatomyositis/immunology Evaluation Studies Fluorescent Antibody Technique, Indirect/statistics & numerical data Humans Immunoenzyme Techniques/*methods/statistics & numerical data Lupus Erythematosus, Systemic/immunology Mixed Connective Tissue Disease/immunology Polymyositis/immunology Scleroderma, Systemic/immunology Sjogren's Syndrome/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 15:20
Dernière modification de la notice
20/08/2019 13:37
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