Corticosterone induces 11 beta-HSD and mineralocorticoid specificity in an amphibian urinary bladder cell line
Détails
ID Serval
serval:BIB_40266D21C0AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Corticosterone induces 11 beta-HSD and mineralocorticoid specificity in an amphibian urinary bladder cell line
Périodique
American Journal of Physiology
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
02/1993
Volume
264
Numéro
2 Pt 1
Pages
C317-22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
We have examined the mineralocorticoid specificity in a TBM 18-23 cell line derived from the toad bladder epithelium. In cells grown on porous substrate, corticosterone was more potent than aldosterone in stimulating a sodium transport response, measured by the short-circuit current method 6 h after hormone addition [mean affinity constant (K0.5) for corticosterone = 1 nM vs. K0.5 for aldosterone = 8 nM]. The time course of effects and saturation kinetics were identical for both agonists, suggesting interaction with identical receptors. Whereas the dose-response relationship for aldosterone did not change with time of incubation (6 vs. 24 h), the dose-response curve for corticosterone became biphasic at 24-h incubation (apparent K0.5 as high as 40 nM), demonstrating that corticosterone became apparently less potent with time. Pretreatment with carbenoxolone, a potent inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), restored full sensitivity at 24-h incubation to corticosterone. The 11 beta-HSD activity was low during the first 3 h of incubation in the presence of 3 nM corticosterone, and only a small fraction (approximately 7%) of corticosterone was metabolized. At 24-h incubation, 11 beta-HSD activity increased approximately 2.5-fold (P < 0.001, n = 8). We conclude that 11 beta-HSD activity is induced by its own substrate in TBM cells in parallel with the induction of the carbenoxolone-sensitive sodium transport response.
Mots-clé
11-beta-Hydroxysteroid Dehydrogenases
Aldosterone/pharmacology
Animals
Binding, Competitive
Biological Transport/drug effects
Bufo marinus
Carbenoxolone/metabolism/pharmacology
Cell Line
Corticosterone/*pharmacology
Dose-Response Relationship, Drug
Epithelium/metabolism
Hydroxysteroid Dehydrogenases/*metabolism
Mineralocorticoids/*metabolism
Sodium/metabolism
Time Factors
Urinary Bladder/cytology/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 14:37