Dermatophyte identification in skin and hair samples using a simple and reliable nested polymerase chain reaction assay.

Détails

ID Serval
serval:BIB_3FEC29973EFA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dermatophyte identification in skin and hair samples using a simple and reliable nested polymerase chain reaction assay.
Périodique
British Journal of Dermatology
Auteur⸱e⸱s
Verrier J., Krähenbühl L., Bontems O., Fratti M., Salamin K., Monod M.
ISSN
1365-2133 (Electronic)
ISSN-L
0007-0963
Statut éditorial
Publié
Date de publication
2013
Volume
168
Numéro
2
Pages
295-301
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
BACKGROUND: Dermatophyte identification in tinea capitis is essential for choosing the appropriate treatment and in tinea infections to identify the possible source. The failure of fungi to grow in cultures frequently occurs, especially in cases of previous antifungal therapy.
OBJECTIVES: To develop a rapid polymerase chain reaction (PCR) sequencing assay for dermatophyte identification in tinea capitis and tinea corporis.
MATERIAL AND METHODS: Fungal DNA was extracted from hair and skin samples that were confirmed to be positive by direct mycological examination. Dermatophytes were identified by the sequence of a 28S ribosomal DNA subunit amplicon generated by nested PCR.
RESULTS: Nested PCR was found to be necessary to obtain amplicons in substantial amounts for dermatophyte identification by sequencing. The results agreed with those of classical mycological identification in 14 of 23, 6 of 10, and 20 of 23 cases of tinea capitis, tinea corporis and tinea pedis, respectively, from which a dermatophyte was obtained in culture. In seven of the 56 cases, another dermatophyte was identified, revealing previous misidentification. A dermatophyte was identified in 12 of 18, three of five, and four of nine cases of tinea capitis, tinea corporis and tinea pedis, respectively, in cases in which no dermatophyte grew in culture.
CONCLUSIONS: Although the gold standard dermatophyte identification from clinical samples remains fungal cultures, the assay developed in the present study is especially suitable for tinea capitis. Improved sensitivity for the identification of dermatophyte species was obtained as it is possible to identify the dermatophyte when the fungus fails to grow in cultures.
Pubmed
Web of science
Création de la notice
21/03/2013 16:37
Dernière modification de la notice
20/08/2019 13:37
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