Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice.

Détails

ID Serval
serval:BIB_3FACAAD80CCB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice.
Périodique
FASEB journal
Auteur(s)
van der Wijk A.E., Wisniewska-Kruk J., Vogels IMC, van Veen H.A., Ip W.F., van der Wel N.N., van Noorden CJF, Schlingemann R.O., Klaassen I.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Statut éditorial
Publié
Date de publication
04/2019
Peer-reviewed
Oui
Volume
33
Numéro
4
Pages
5320-5333
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Insight into the molecular and cellular processes in blood-retinal barrier (BRB) development, including the contribution of paracellular and transcellular pathways, is still incomplete but may help to understand the inverse process of BRB loss in pathologic eye conditions. In this comprehensive observational study, we describe in detail the formation of the BRB at the molecular level in physiologic conditions, using mice from postnatal day (P)3 to P25. Our data indicate that immature blood vessels already have tight junctions at P5, before the formation of a functional BRB. Expression of the endothelial cell-specific protein plasmalemma vesicle-associated protein (PLVAP), which is known to be involved in transcellular transport and associated with BRB permeability, decreased during development and was absent when a functional barrier was formed. Moreover, we show that PLVAP deficiency causes a transient delay in retinal vascular development and changes in mRNA expression levels of endothelial permeability pathway proteins.-Van der Wijk, A.-E., Wisniewska-Kruk, J., Vogels, I. M. C., van Veen, H. A., Ip, W. F., van der Wel, N. N., van Noorden, C. J. F., Schlingemann, R. O., Klaassen, I. Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice.
Mots-clé
Animals, Blood-Retinal Barrier/embryology, Blood-Retinal Barrier/metabolism, Blood-Retinal Barrier/ultrastructure, Blotting, Western, Exons/genetics, Gene Expression Regulation, Developmental, Genotype, Humans, Membrane Proteins/genetics, Membrane Proteins/metabolism, Mice, Mice, Mutant Strains, Microscopy, Electron, Transmission, RNA, Messenger/genetics, RNA, Messenger/metabolism, Transcriptome, VEGF signaling, tight junctions, transcellular permeability
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/02/2019 12:05
Dernière modification de la notice
23/01/2020 7:19
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