The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata

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Ressource 1Télécharger: Thiébaut et al. - 2017 - The CCAAT-Binding Complex Controls Respiratory Gen.pdf (2800.72 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_3F9D0F6B6363
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
Périodique
Scientific Reports
Auteur(s)
Thiébaut Antonin, Delaveau Thierry, Benchouaia Médine, Boeri Julia, Garcia Mathilde, Lelandais Gaëlle, Devaux Frédéric
ISSN
2045-2322
Statut éditorial
Publié
Date de publication
06/2017
Volume
7
Numéro
1
Pages
3531
Langue
anglais
Résumé
The CCAAT-binding complex (CBC) is a heterotrimeric transcription factor which is widely conserved in eukaryotes. In the model yeast S. cerevisiae, CBC positively controls the expression of respiratory pathway genes. This role involves interactions with the regulatory subunit Hap4. In many pathogenic fungi, CBC interacts with the HapX regulatory subunit to control iron homeostasis. HapX is a bZIP protein which only shares with Hap4 the Hap4Like domain (Hap4L) required for its interaction with CBC. Here, we show that CBC has a dual role in the pathogenic yeast C. glabrata. It is required, along with Hap4, for the constitutive expression of respiratory genes and it is also essential for the iron stress response, which is mediated by the Yap5 bZIP transcription factor. Interestingly, Yap5 contains a vestigial Hap4L domain. The mutagenesis of this domain severely reduced Yap5 binding to its targets and compromised its interaction with Hap5. Hence, Yap5, like HapX in other species, acts as a CBC regulatory subunit in the regulation of iron stress response. This work reveals new aspects of iron homeostasis in C. glabrata and of the evolution of the role of CBC and Hap4L-bZIP proteins in this process.
Création de la notice
22/01/2020 14:58
Dernière modification de la notice
23/01/2020 7:26
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