Synergic effects of tactolimus and azole antifungal agents against azole-resistant Candida albican strains.

Détails

ID Serval
serval:BIB_3F336DCD33F8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Synergic effects of tactolimus and azole antifungal agents against azole-resistant Candida albican strains.
Périodique
Journal of Antimicrobial Chemotherapy
Auteur(s)
Maesaki S., Marichal P., Hossain M.A., Sanglard D., Vanden Bossche H., Kohno S.
ISSN
0305-7453 (Print)
ISSN-L
0305-7453
Statut éditorial
Publié
Date de publication
12/1998
Peer-reviewed
Oui
Volume
42
Numéro
6
Pages
747-753
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
We investigated the effects of combining tacrolimus and azole antifungal agents in azole-resistant strains of Candida albicans by comparing the accumulation of [3H]itraconazole. The CDR1-expressing resistant strain C26 accumulated less itraconazole than the CaMDR-expressing resistant strain C40 or the azole-sensitive strain B2630. A CDR1-expressing Saccharomyces cerevisiae mutant, DSY415, showed a marked reduction in the accumulation of both fluconazole and itraconazole. A CaMDR-expressing S. cerevisiae mutant, DSY416, also showed lower accumulation of fluconazole, but not of itraconazole. The addition of sodium azide, an electron-transport chain inhibitor, increased the intracellular accumulation of itraconazole only in the C26 strain, and not in the C40 or B2630 strains. Addition of tacrolimus, an inhibitor of multidrug resistance proteins, resulted in the highest increase in itraconazole accumulation in the C26 strain. The combination of itraconazole and tacrolimus was synergic in azole-resistant C. albicans strains. In the C26 strain, the MIC of itraconazole decreased from >8 to 0.5 mg/L when combined with tacrolimus. Our results showed that two multidrug resistance phenotypes (encoded by the CDR1 and CaMDR genes) in C. albicans have different substrate specificity for azole antifungal agents and that a combination of tacrolimus and azole antifungal agents is effective against azole-resistant strains of C. albicans.
Mots-clé
AIDS-Related Opportunistic Infections/microbiology, Antifungal Agents/pharmacology, Azoles/pharmacology, Candida albicans/drug effects, Candida albicans/growth & development, Candidiasis, Oral/microbiology, Drug Resistance, Microbial/genetics, Drug Resistance, Multiple/genetics, Drug Synergism, Fluconazole/metabolism, Fungal Proteins/genetics, Gene Expression Regulation, Fungal, Genes, MDR/genetics, Humans, Itraconazole/antagonists & inhibitors, Itraconazole/metabolism, Membrane Transport Proteins, Microbial Sensitivity Tests, Tacrolimus/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:40
Dernière modification de la notice
20/08/2019 14:36
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