Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing
Détails
ID Serval
serval:BIB_3EF31B0197C4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing
Périodique
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
07/1987
Volume
84
Numéro
13
Pages
4596-600
Notes
Comparative Study
Journal Article --- Old month value: Jul
Journal Article --- Old month value: Jul
Résumé
Thirty-four lymphoblastoid cell lines that had been previously typed for HLA-DP antigens by primed lymphocyte typing (PLT) were tested by Southern blotting and by ELISA. Using two DP beta probes and a DP alpha probe with a series of enzymes, it is possible to identify restriction fragment length polymorphism (RFLP) patterns characteristic of DPw1, -2, -3, -4, and possibly -5. ELISA typing results, based on two polymorphic DP antibodies DP11.1 and ILR1, were compared with PLT-defined and RFLP-defined types. Thus, using a range of probes and enzymes it is possible to identify DP polymorphism. The value of monoclonal antibodies for such studies is demonstrated, and the molecular data can, in some cases, pinpoint the amino acids responsible for the specificity of the monoclonal antibodies.
Mots-clé
Alleles
Antibodies, Monoclonal/*immunology
Antibody Specificity
Cell Line
DNA/genetics
*DNA, Recombinant
Enzyme-Linked Immunosorbent Assay
*Genetic Markers
HLA-D Antigens/*genetics
HLA-DP Antigens/*genetics/immunology
Histocompatibility Testing/*methods
Humans
Lymphocytes/*immunology
*Polymorphism, Genetic
*Polymorphism, Restriction Fragment Length
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 9:38
Dernière modification de la notice
20/08/2019 14:35