Synthesis and processing of pro-ocytocin in bovine corpus luteum and granulosa cells

Détails

ID Serval
serval:BIB_3E9A49CE83D3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Synthesis and processing of pro-ocytocin in bovine corpus luteum and granulosa cells
Périodique
Molecular and Cellular Endocrinology
Auteur(s)
Camier  M., Benveniste  D., Barre  N., Brakch  N., Cohen  P.
ISSN
0303-7207 (Print)
Statut éditorial
Publié
Date de publication
05/1991
Volume
77
Numéro
1-3
Pages
141-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Bovine corpus luteum is the site of intense production of pro-ocytocin-neurophysin mRNA at day 1 after estrus (Ivell et al. (1985) FEBS Lett. 190, 263-267) which is followed by apparent delayed production of ocytocin. Therefore it is a good model to study both the translational and post-translational production of this neuropeptide in non-hypothalamic tissues and its regulation. In order to assess if this mRNA is translated during the lag period we have analyzed the neurophysin-like species produced in this organ. As early as day 2 after estrus one neurophysin species (pI approximately 4.7) could be detected and was unequivocally identified as pro-ocytocin-neurophysin. In primary cultures of luteinizing granulosa cells, biosynthetic intermediates were characterized, i.e. ocytocin-Gly, ocytocin-Gly-Lys and ocytocin-Gly-Lys-Arg, whereas amidated, fully mature, ocytocin was undetectable. We conclude that translation of pro-ocytocin-neurophysin mRNA takes place soon after transcription and we propose that incomplete processing could be responsible for the low level of ocytocin in the early bovine corpus luteum.
Mots-clé
Amino Acid Sequence Animals Blotting, Western Cattle Cells, Cultured Chromatography, High Pressure Liquid Corpus Luteum/*metabolism Female Granulosa Cells/*metabolism Molecular Sequence Data Neurophysins/metabolism Oxytocin/biosynthesis/*metabolism Protein Precursors/biosynthesis/*metabolism *Protein Processing, Post-Translational
Pubmed
Web of science
Création de la notice
28/01/2008 10:35
Dernière modification de la notice
20/08/2019 13:35
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