Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice
Détails
ID Serval
serval:BIB_3E06610C0261
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice
Périodique
Journal of Immunology
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
12/1985
Volume
135
Numéro
6
Pages
3704-11
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Mice bearing the recessive gene lpr develop an autoimmune syndrome associated with a massive lymphadenopathy, both of which are age and thymus dependent. The predominant accumulating cells in lymphoid tissue of lpr/lpr mice are Thy-1+ but express neither of the mature T cell markers, Lyt-2 or L3T4. We have purified this Lyt-2-/L3T4- subset and examined its phenotype. These cells are not actively cycling, do not express interleukin-2 (IL 2) receptors nor significant levels of antigen receptor, but do express the B cell marker B220. In vitro growth conditions were examined for the lpr Lyt-2-/L3T4- subset. By using a combination of phorbol ester and IL 2, these cells acquired transient expression of IL 2 receptors and grew in an IL 2-dependent manner. Furthermore, these proliferating cells underwent differentiation to a more mature T cell phenotype, with loss of cell surface B220 and acquisition, by a portion, of antigen receptor and Lyt-2. The possible parallels with normal T cell maturation are discussed.
Mots-clé
Animals
*Antigens, Ly/genetics
*Antigens, Surface/genetics
Cell Cycle/drug effects
Cell Differentiation/drug effects
Concanavalin A/pharmacology
Lymph Nodes/cytology
*Lymphocyte Activation/drug effects
Lymphocyte Function-Associated Antigen-1
Mice
Mice, Inbred C57BL/*genetics
Phenotype
Receptors, Immunologic/metabolism
Receptors, Interleukin-2
T-Lymphocytes, Cytotoxic/*classification/cytology/immunology/metabolism
Tetradecanoylphorbol Acetate/pharmacology
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 14:34