Differential expression of perforin in muscle-infiltrating T cells in polymyositis and dermatomyositis

Détails

ID Serval
serval:BIB_3DFADEB3112E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential expression of perforin in muscle-infiltrating T cells in polymyositis and dermatomyositis
Périodique
Journal of Clinical Investigation
Auteur⸱e⸱s
Goebels  N., Michaelis  D., Engelhardt  M., Huber  S., Bender  A., Pongratz  D., Johnson  M. A., Wekerle  H., Tschopp  J., Jenne  D., Hohlfeld  R.
ISSN
0021-9738 (Print)
Statut éditorial
Publié
Date de publication
06/1996
Volume
97
Numéro
12
Pages
2905-10
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 15
Résumé
Polymyositis (PM) and dermatomyositis (DM) are the prototypical inflammatory diseases of skeletal muscle. In PM, CD8+ T cells invade and destroy muscle fibers, whereas humoral effector mechanisms prevail in DM. We studied the expression of the cytotoxic mediator perforin in inflammatory cells in PM and DM muscle by semiquantitative PCR, immunohistochemistry and confocal laser microscopy. Similar levels of perforin mRNA were expressed in PM and DM, and abundant perforin-expressing CD3+CD8+ and CD3+ CD4+ T cells were observed in both diseases. However, there was a striking difference in the intracellular localization of perforin. In DM, perforin was distributed randomly in the cytoplasm of the inflammatory T cells. In contrast, 43% of the CD8+ T cells that contacted a muscle fiber in PM showed perforin located vectorially towards the target muscle fiber. The results suggest (a) that the random distribution of perforin in the cytoplasm of muscle-infiltrating T cells observed in DM reflects nonspecific activation, and (b) that the vectorial orientation observed only in PM reflects the specific recognition via the T cell receptor of an antigen on the muscle fiber surface, pointing to a perforin- and secretion-dependent mechanism of muscle fiber injury.
Mots-clé
Base Sequence Dermatomyositis/*metabolism/pathology Humans Membrane Glycoproteins/*analysis Molecular Sequence Data Muscles/*chemistry/pathology Polymerase Chain Reaction Polymyositis/*metabolism/pathology Pore Forming Cytotoxic Proteins T-Lymphocytes/*chemistry
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:19
Dernière modification de la notice
20/08/2019 13:34
Données d'usage