A syngeneic monoclonal anti-idiotype antibody against HNK-1: characterization and cytotoxic activity in vitro

Détails

ID Serval
serval:BIB_3DEE21780E40
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A syngeneic monoclonal anti-idiotype antibody against HNK-1: characterization and cytotoxic activity in vitro
Périodique
Clinical and Experimental Immunology
Auteur(s)
Schluep  M., Page  N., Burger  D., Steck  A. J.
ISSN
0009-9104 (Print)
Statut éditorial
Publié
Date de publication
02/1989
Volume
75
Numéro
2
Pages
301-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
A syngeneic, monoclonal, anti-idiotype antibody, G6D9, was raised against the mouse monoclonal IgM HNK-1. G6D9, characterized as an IgG1-kappa, inhibits the binding of HNK-1 to the myelin-associated glycoprotein (MAG). G6D9 does not interfere with the binding of various human monoclonal anti-MAG IgM to their specific antigen. G6D9 binds HNK-1 hybridoma cells on their surface and within their cytoplasm, as demonstrated using indirect immunofluorescence. In the presence of complement, G6D9 is cytotoxic for HNK-1-secreting cells. A cell lysis of 32% was observed and compared to lysis obtained with other antibodies directed against mouse lymphocyte antigens. The use of G6D9 as a tool to study specific cytotoxicity and immunotherapy in vivo is discussed.
Mots-clé
Animals Antibodies, Anti-Idiotypic/*immunology Antibodies, Monoclonal/*immunology Antibody Specificity Complement System Proteins/immunology *Cytotoxicity, Immunologic Fluorescent Antibody Technique Humans Immunoglobulin G/immunology Immunoglobulin Idiotypes/*immunology Immunoglobulin M/immunology Mice Mice, Inbred Strains
Pubmed
Web of science
Création de la notice
25/01/2008 13:46
Dernière modification de la notice
20/08/2019 14:34
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