A positive signal is transduced via surface CD4 molecules

Détails

ID Serval
serval:BIB_3D1B467A7E9E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A positive signal is transduced via surface CD4 molecules
Périodique
Research in Immunology
Auteur⸱e⸱s
Carrel  S., Lamarre  D., Isler  P., Rapin  C., Fleury  S., Salvi  S., Sekaly  R. P., Cerottini  J. C.
ISSN
0923-2494 (Print)
Statut éditorial
Publié
Date de publication
08/1989
Volume
140
Numéro
5-6
Pages
545-61
Notes
In Vitro
Journal Article --- Old month value: Jun-Aug
Résumé
We have previously reported on the identification of a monoclonal antibody (mAb) directed against the human CD4 antigen which is capable of activating CD4+ peripheral blood T cells in the absence of other stimuli. In the present study, we extended these findings by demonstrating that the mAb, termed B66, was able to induce the production of interleukin-2 in murine T-cell hybridoma transfectants expressing the human CD4 glycoprotein. Moreover, we found that incubation of Jurkat cells with mAb B66 resulted in the nearly complete disappearance of both CD4 and CD3 from the cell surface, whereas modulation of CD4, but not CD3, was observed after incubation with a non-stimulatory anti-CD4 mAb. Similar results were obtained in modulation experiments using human CD4-expressing murine transfectants. It is thus conceivable that the stimulatory activity of anti-CD4 mAb B66 may be associated with an effect on the CD3 molecular complex. While the biochemical basis for the unique stimulatory activity of mAb B66 has yet to be defined, these findings provide direct evidence that cross-linking of CD4 alone may cause T-cell activation, thus supporting the notion that this glycoprotein can transduce independent positive signals upon binding to class II major histocompatibility complex molecules.
Mots-clé
Antibodies, Monoclonal Antigens, CD3 *Antigens, Differentiation, T-Lymphocyte Binding, Competitive Epitopes Humans Lymphocyte Activation Receptors, Antigen, T-Cell *Signal Transduction T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 11:14
Dernière modification de la notice
20/08/2019 13:33
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