Trachea, lung, and tracheobronchial lymph nodes are the major sites where antigen-presenting cells are detected after nasal vaccination of mice with human papillomavirus type 16 virus-like particles.

Détails

ID Serval
serval:BIB_3C8F1934B1D4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Trachea, lung, and tracheobronchial lymph nodes are the major sites where antigen-presenting cells are detected after nasal vaccination of mice with human papillomavirus type 16 virus-like particles.
Périodique
Journal of Virology
Auteur⸱e⸱s
Balmelli C., Demotz S., Acha-Orbea H., De Grandi P., Nardelli-Haefliger D.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
76
Numéro
24
Pages
12596-12602
Langue
anglais
Résumé
Vaccination by the nasal route has been successfully used for the induction of immune responses. Either the nasal-associated lymphoid tissue (NALT), the bronchus-associated lymphoid tissue, or lung dendritic cells have been mainly involved. Following nasal vaccination of mice with human papillomavirus type 16 (HPV16) virus-like-particles (VLPs), we have previously shown that interaction of the antigen with the lower respiratory tract was necessary to induce high titers of neutralizing antibodies in genital secretions. However, following a parenteral priming, nasal vaccination with HPV16 VLPs did not require interaction with the lung to induce a mucosal immune response. To evaluate the contribution of the upper and lower respiratory tissues and associated lymph nodes (LN) in the induction of humoral responses against HPV16 VLPs after nasal vaccination, we localized the immune inductive sites and identified the antigen-presenting cells involved using a specific CD4(+) T-cell hybridoma. Our results show that the trachea, the lung, and the tracheobronchial LN were the major sites responsible for the induction of the immune response against HPV16 VLP, while the NALT only played a minor role. Altogether, our data suggest that vaccination strategies aiming to induce efficient immune responses against HPV16 VLP in the female genital tract should target the lower respiratory tract.
Mots-clé
Administration, Intranasal, Animals, Antigen-Presenting Cells/immunology, B-Lymphocytes/immunology, B-Lymphocytes/virology, Bronchi/immunology, Capsid Proteins, Cloning, Molecular, Dendritic Cells/immunology, Dendritic Cells/virology, Female, Hybridomas/immunology, Lung/immunology, Lymph Nodes/immunology, Mice, Mice, Inbred BALB C, Oncogene Proteins, Viral/immunology, Papillomaviridae/immunology, Trachea/immunology, Vaccination, Viral Vaccines/administration & dosage, Viral Vaccines/immunology, Virion/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:48
Dernière modification de la notice
20/08/2019 13:32
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