Chromosomal number aberrations and transformation in adult mouse retinal stem cells in vitro.

Détails

ID Serval
serval:BIB_3C7B4D3A0834
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Chromosomal number aberrations and transformation in adult mouse retinal stem cells in vitro.
Périodique
Investigative Ophthalmology & Visual Science
Auteur⸱e⸱s
Djojosubroto Meta, Bollotte Frederic, Wirapati Pratyaksha, Radtke Freddy, Stamenkovic Ivan, Arsenijevic Yvan
ISSN
1552-5783[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
50
Numéro
12
Pages
5975-5987
Langue
anglais
Résumé
PURPOSE: The potential of stem cells (SCs) as a source for cell-based therapy on a wide range of degenerative diseases and damaged tissues such as retinal degeneration has been recognized. Generation of a high number of retinal stem cells (RSCs) in vitro would thus be beneficial for transplantation in the retina. However, as cells in prolonged cultivation may be unstable and thus have a risk of transformation, it is important to assess the stability of these cells. METHODS: Chromosomal aberrations were analyzed in mouse RSC lines isolated from adult and from postnatal day (PN)1 mouse retinas. Moreover, selected cell lines were tested for anchorage-dependent proliferation, and SCs were transplanted into immunocompromised mice to assess the possibility of transformation. RESULTS: Marked aneuploidy occurred in all adult cell lines, albeit to different degrees, and neonatal RSCs were the most stable and displayed a normal karyotype until at least passage 9. Of interest, the level of aneuploidy of adult RSCs did not necessarily correlate with cell transformation. Only the adult RSC lines passaged for longer periods and with a higher dilution ratio underwent transformation. Furthermore, we identified several cell cycle proteins that might support the continuous proliferation and transformation of the cells. CONCLUSIONS: Adult RSCs rapidly accumulated severe chromosomal aberrations during cultivation, which led to cell transformation in some cell lines. The culture condition plays an important role in supporting the selection and growth of transformed cells.
Mots-clé
Tumor Suppression, P53-Independent Pathway, Differential Regulation, Photoreceptor Fate, Progenitor Cells, Mammalian Eye, Embryo Cells, Ink4a Locus, Dna-Damage, P53
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/12/2009 15:02
Dernière modification de la notice
20/08/2019 13:32
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