Human papillomavirus type 16 virus-like particles expressed in attenuated Salmonella typhimurium elicit mucosal and systemic neutralizing antibodies in mice

Détails

ID Serval
serval:BIB_3C772EDCCED4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human papillomavirus type 16 virus-like particles expressed in attenuated Salmonella typhimurium elicit mucosal and systemic neutralizing antibodies in mice
Périodique
Infection and Immunity
Auteur(s)
Nardelli-Haefliger  D., Roden  R. B., Benyacoub  J., Sahli  R., Kraehenbuhl  J. P., Schiller  J. T., Lachat  P., Potts  A., De Grandi  P.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
08/1997
Volume
65
Numéro
8
Pages
3328-36
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
Attenuated strains of Salmonella are attractive live vaccine candidates for eliciting mucosal as well as systemic immune responses. The ability to induce immune responses in the reproductive tract may be critical for the effectiveness of a prophylactic vaccine against genital human papillomaviruses (HPV), which are important etiologic agents in the development of cervical cancer. To examine the potential of a live Salmonella-based vaccine to prevent genital HPV infection, the L1 major capsid protein from HPV type 16 (HPV16) was constitutively expressed in the PhoPc strain of Salmonella typhimurium. As demonstrated by electron microscopy, the L1 protein expressed in these bacteria assembled into virus-like particles (VLPs) that resemble authentic papillomavirus virions. This is the first demonstration that papillomavirus VLPs can self-assemble in prokaryotes. BALB/c mice were immunized with the HPV16 L1 recombinant PhoPc strain by the oral and nasal routes. Despite a low stability of the L1-expressing plasmid in vivo, a double nasal immunization was effective in inducing L1-specific serum antibodies that recognized mainly native, but not disassembled, VLPs. These antibodies effectively neutralized HPV16 pseudotyped virions in an in vitro infectivity assay. Conformationally dependent anti-VLP immunoglobulin A (IgA) and IgG were also detected in oral and vaginal secretions, indicating that potentially protective antibody responses were elicited at mucosal sites. Recombinant attenuated Salmonella expressing HPV capsids may represent a promising vaccine candidate against genital HPV infection.
Mots-clé
Animals Antibodies, Viral/*analysis Epitopes Female Genital Diseases, Female/prevention & control Immune Sera/immunology Immunity, Mucosal Immunization Mice Mice, Inbred BALB C Papillomaviridae/*immunology Papillomavirus Infections/prevention & control Plasmids Salmonella typhimurium/*genetics Tumor Virus Infections/prevention & control Vaccines, Attenuated/immunology Vaccines, Synthetic/*immunology Viral Vaccines/*immunology Virion/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 14:32
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