Improved efficiency of a Salmonella-based vaccine against human papillomavirus type 16 virus-like particles achieved by using a codon-optimized version of L1.

Détails

ID Serval
serval:BIB_3C646ABDCED9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improved efficiency of a Salmonella-based vaccine against human papillomavirus type 16 virus-like particles achieved by using a codon-optimized version of L1.
Périodique
Journal of Virology
Auteur⸱e⸱s
Baud D., Ponci F., Bobst M., De Grandi P., Nardelli-Haefliger D.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
78
Numéro
23
Pages
12901-12909
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Cervical cancer results from cervical infection by human papillomaviruses (HPVs), especially HPV16. An effective vaccine against these HPVs is expected to have a dramatic impact on the incidence of this cancer and its precursor lesions. The leading candidate, a subunit prophylactic HPV virus-like particle (VLP) vaccine, can protect women from HPV infection. An alternative improved vaccine that avoids parenteral injection, that is efficient with a single dose, and that induces mucosal immunity might greatly facilitate vaccine implementation in different settings. In this study, we have constructed a new generation of recombinant Salmonella organisms that assemble HPV16 VLPs and induce high titers of neutralizing antibodies in mice after a single nasal or oral immunization with live bacteria. This was achieved through the expression of a HPV16 L1 capsid gene whose codon usage was optimized to fit with the most frequently used codons in Salmonella. Interestingly, the high immunogenicity of the new recombinant bacteria did not correlate with an increased expression of L1 VLPs but with a greater stability of the L1-expressing plasmid in vitro and in vivo in absence of antibiotic selection. Anti-HPV16 humoral and neutralizing responses were also observed with different Salmonella enterica serovar Typhimurium strains whose attenuating deletions have already been shown to be safe after oral vaccination of humans. Thus, our findings are a promising improvement toward a vaccine strain that could be tested in human volunteers.
Mots-clé
Animals, Antibodies, Viral/blood, Base Sequence, Capsid Proteins/genetics, Capsid Proteins/immunology, Codon, Female, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Oncogene Proteins, Viral/genetics, Oncogene Proteins, Viral/immunology, Papillomaviridae/immunology, Papillomavirus Vaccines, Salmonella/genetics, Vaccination, Vaccines, Synthetic/immunology, Viral Vaccines/immunology, Virion/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:40
Dernière modification de la notice
20/08/2019 13:32
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