Fas ligand-induced proinflammatory transcriptional responses in reconstructed human epidermis. Recruitment of the epidermal growth factor receptor and activation of MAP kinases.
Détails
Télécharger: BIB_3B7780E4D07A.P001.pdf (735.64 [Ko])
Etat: Public
Version: de l'auteur⸱e
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_3B7780E4D07A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fas ligand-induced proinflammatory transcriptional responses in reconstructed human epidermis. Recruitment of the epidermal growth factor receptor and activation of MAP kinases.
Périodique
Journal of Biological Chemistry
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
2008
Volume
283
Numéro
2
Pages
919-928
Langue
anglais
Résumé
Fas ligand (FasL) exerts potent proapoptotic and proinflammatory actions on epidermal keratinocytes and has been implicated in the pathogenesis of eczema, toxic epidermal necrolysis, and drug-induced skin eruptions. We used reconstructed human epidermis to investigate the mechanisms of FasL-induced inflammatory responses and their relationships with FasL-triggered caspase activity. Caspase activity was a potent antagonist of the pro-inflammatory gene expression triggered by FasL prior to the onset of cell death. Furthermore, we found that FasL-stimulated autocrine production of epidermal growth factor receptor (EGFR) ligands, and the subsequent activation of EGFR and ERK1 and ERK2 mitogen-activated protein kinases, were obligatory extracellular steps for the FasL-induced expression of a subset of inflammatory mediators, including CXCL8/interleukin (IL)-8, ICAM-1, IL-1alpha, IL-1beta, CCL20/MIP-3alpha, and thymic stromal lymphopoietin. These results expand the known physiological role of EGFR and its ligands from promoting keratinocyte mitogenesis and survival to mediating FasL-induced epidermal inflammation.
Mots-clé
Apoptosis, Cell Line, DNA Primers, Enzyme Activation, Epidermis/physiopathology, Extracellular Signal-Regulated MAP Kinases/genetics, Extracellular Signal-Regulated MAP Kinases/metabolism, Fas Ligand Protein/physiology, Humans, Infant, Newborn, Inflammation/physiopathology, Keratinocytes/physiology, Kidney, Mitogen-Activated Protein Kinase 3/genetics, Mitogen-Activated Protein Kinase 3/metabolism, Oligonucleotide Array Sequence Analysis, RNA Interference, RNA, Messenger/genetics, RNA, Small Interfering/genetics, Receptor, Epidermal Growth Factor/physiology, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/01/2008 18:30
Dernière modification de la notice
20/08/2019 14:31