Mesenchymal stem cells rescue CD34+ cells from radiation-induced apoptosis and sustain hematopoietic reconstitution after coculture and cografting in lethally irradiated baboons: is autologous stem cell therapy in nuclear accident settings hype or reality?
Détails
ID Serval
serval:BIB_3B3B9D3055FC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mesenchymal stem cells rescue CD34+ cells from radiation-induced apoptosis and sustain hematopoietic reconstitution after coculture and cografting in lethally irradiated baboons: is autologous stem cell therapy in nuclear accident settings hype or reality?
Périodique
Bone Marrow Transplant
ISSN
0268-3369 (Print)
ISSN-L
0268-3369
Statut éditorial
Publié
Date de publication
06/2005
Volume
35
Numéro
12
Pages
1201-9
Langue
anglais
Notes
Drouet, M
Mourcin, F
Grenier, N
Delaunay, C
Mayol, J F
Lataillade, J J
Peinnequin, A
Herodin, F
eng
Research Support, Non-U.S. Gov't
England
2005/04/12
Bone Marrow Transplant. 2005 Jun;35(12):1201-9. doi: 10.1038/sj.bmt.1704970.
Mourcin, F
Grenier, N
Delaunay, C
Mayol, J F
Lataillade, J J
Peinnequin, A
Herodin, F
eng
Research Support, Non-U.S. Gov't
England
2005/04/12
Bone Marrow Transplant. 2005 Jun;35(12):1201-9. doi: 10.1038/sj.bmt.1704970.
Résumé
Autologous stem cell therapy (ACT) has been proposed to prevent irradiated victims from bone marrow (BM) aplasia by grafting hematopoietic stem and progenitor cells (HSPCs) collected early after damage, provided that a functional graft of sufficient size could be produced ex vivo. To address this issue, we set up a baboon model of cell therapy in which autologous peripheral blood HSPCs collected before lethal total body irradiation were irradiated in vitro (2.5 Gy, D0 1 Gy) to mimic the cell damage, cultured in small numbers for a week in a serum-free medium in the presence of antiapoptotic cytokines and mesenchymal stem cells (MSCs) and then cografted. Our study shows that baboons cografted with expanded cells issued from 0.75 and 1 x 10(6)/kg irradiated CD34+ cells and MSCs (n=2) exhibited a stable long-term multilineage engraftment. Hematopoietic recovery became uncertain when reducing the CD34+ cell input (0.4 x 10(6)/kg CD34+ cells; n=3). However, platelet recovery was accelerated in all surviving cografted animals, when compared with baboons transplanted with unirradiated, unmanipulated CD34+ cells (0.5-1 x 10(6)/kg, n=4). Baboons grafted with MSCs alone (n=3) did not recover. In all cases, the nonhematopoietic toxicity remained huge. This baboon study suggests that ACT feasibility is limited.
Mots-clé
Animals, Antigens, CD34, Apoptosis/radiation effects, Cell Communication, Cell Culture Techniques, Coculture Techniques, Graft Survival, *Hematopoiesis, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells/*cytology/radiation effects, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells/cytology/*physiology, Models, Animal, Papio, Radiation Injuries/*therapy, Radioactive Hazard Release, Stem Cell Transplantation/*methods, Transplantation, Autologous, Whole-Body Irradiation
Pubmed
Création de la notice
02/05/2024 9:41
Dernière modification de la notice
28/05/2024 6:10