Activation of CD4+ and CD8+ lymphocyte subsets by streptozotocin in murine popliteal lymph node (PLN) test.

Détails

ID Serval
serval:BIB_3B0FB8B5E3CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Activation of CD4+ and CD8+ lymphocyte subsets by streptozotocin in murine popliteal lymph node (PLN) test.
Périodique
Journal of Autoimmunity
Auteur(s)
Krzystyniak K., Brouland J.P., Panaye G., Patriarca C., Verdier F., Descotes J., Revillard J.P.
ISSN
0896-8411 (Print)
ISSN-L
0896-8411
Statut éditorial
Publié
Date de publication
1992
Peer-reviewed
Oui
Volume
5
Numéro
2
Pages
183-197
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Time-related and dose-related popliteal lymph node (PLN) enlargement and lymphocyte subset alterations owing to subcutaneous (s.c.) injection of streptozotocin (STZ) into the foot pad were determined in (C57BL/6 x DBA/2)F1 [B6D2F1] mice. Early cell activation and time-related changes in T and B lymphocyte subsets were monitored during the onset of STZ-induced lymphoproliferative reaction by flow cytometry and immunophenotyping of lymphocyte subsets stained with a panel of monoclonal antibodies. Examination of cell size and chromatin decondensing for T and B cell subsets revealed differences in their activation profiles during the early phase of STZ-induced lymph node enlargement. The kinetics of the reaction showed initial activation and proliferation of CD4+ cells associated with accumulation of CD8+ and B cells. Subsequently CD8+ cells were activated and proliferated, but there was no evidence of early or late B cell activation as shown by the lack of increase in cell size or nuclear decondensation and the low and transient synthesis of immunoglobulins. The activation characteristics for CD4+ and CD8+ cell subsets in STZ-induced node enlargement were found to be analogous with T cell activation in acute allogeneic graft-versus-host (GVH) reaction in which the F1 recipient differed from the parent at both class I and II MHC loci. Our data support a central role for T cell activation in the induction of STZ-related PLN enlargement and suggest that recirculatory host B cells can play a major role in early node enlargement.
Mots-clé
Animals, Antibody Formation/drug effects, Antigens, Surface/analysis, Autoimmune Diseases/chemically induced, Autoimmune Diseases/immunology, CD4-Positive T-Lymphocytes/drug effects, CD4-Positive T-Lymphocytes/immunology, Female, Lymph Nodes/drug effects, Lymph Nodes/immunology, Lymphadenitis/chemically induced, Lymphadenitis/immunology, Lymphocyte Activation/drug effects, Mice, Mice, Inbred C57BL/immunology, Mice, Inbred DBA/immunology, Streptozocin/pharmacology, Streptozocin/toxicity, T-Lymphocytes, Regulatory/drug effects, T-Lymphocytes, Regulatory/immunology
Pubmed
Web of science
Création de la notice
13/10/2015 9:55
Dernière modification de la notice
20/08/2019 14:30
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