The POU domain is a bipartite DNA-binding structure.

Détails

ID Serval
serval:BIB_3AB66456401F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The POU domain is a bipartite DNA-binding structure.
Périodique
Nature
Auteur⸱e⸱s
Sturm R.A., Herr W.
ISSN
0028-0836
Statut éditorial
Publié
Date de publication
12/1988
Peer-reviewed
Oui
Volume
336
Numéro
6199
Pages
601-604
Langue
anglais
Résumé
The POU domain (pronounced 'pow') is a highly charged 155-162-amino-acid (aa) region of sequence similarity contained within three mammalian transcription factors. Pt-1 (ref. 2), Oct-1 (ref. 3) and Oct-2 (ref. 4), and the product of the nematode gene unc-86 (ref. 5) which is involved in determining neural cell lineage. This domain consists of two subdomains, a C-terminal homoeo domain and an N-terminal POU-specific region separated by a short nonconserved linker; the sequence relationship shows that the POU homoeo domains form a distinct POU-related family. In the ubiquitous and lymphoid-specific octamer-motif binding proteins Oct-1 and Oct-2, the POU domain is sufficient for sequence-specific DNA binding. Homoeobox domains contain a helix-turn-helix DNA-binding motif, first identified in bacterial repressors. The helix-turn-helix region of the POU domain is important for DNA binding and, in other classes of homoeo-containing proteins, the entire homoeo domain is sufficient for DNA binding; thus the new POU-specific region could be involved in other functions such as protein-protein interactions. Nevertheless, we show here that in fact the POU domain is a novel bipartite DNA-binding structure in which the POU homoeo and POU-specific regions form two subdomains that are both required for DNA binding but are held together by a flexible linker.
Mots-clé
Base Sequence, DNA, Genes, Homeobox, Genes, Viral, Molecular Sequence Data, Mutation, Simian virus 40, Simplexvirus, Transcription Factors
Pubmed
Web of science
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 13:30
Données d'usage