Thymocyte stimulation by anti-TCR-beta, but not by anti-TCR-alpha, leads to induction of developmental transcription program.

Détails

ID Serval
serval:BIB_3A23626F4B80
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Thymocyte stimulation by anti-TCR-beta, but not by anti-TCR-alpha, leads to induction of developmental transcription program.
Périodique
Journal of Leukocyte Biology
Auteur(s)
Niederberger N., Buehler L.K., Ampudia J., Gascoigne N.R.
ISSN
0741-5400 (Print)
ISSN-L
0741-5400
Statut éditorial
Publié
Date de publication
2005
Volume
77
Numéro
5
Pages
830-841
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Anti-T cell receptor (aTCR) antibody (Ab) stimulation of T cells results in TCR down-modulation and T cell activation. Differences in the effect of anti-alpha-chain and beta-chain Ab have been reported on thymocytes. Anti-beta-chain Ab but not anti-alpha-chain reagents cause long-term TCR down-modulation. However, both types of Ab result in TCR cross-linking and activate early steps in signal transduction. In this study, we show that TCR internalization and calcium flux, hallmarks of T cell activation, are similar with aValpha and aVbeta treatment. Therefore, we have compared the gene expression profiles of preselection thymocytes stimulated with these reagents. We find that aValpha treatment does not cause any significant change in gene expression compared with control culture conditions. In contrast, aVbeta stimulation results in numerous changes in gene expression. The alterations of expression of genes known to be expressed in thymocytes are similar to changes caused by positive thymic selection, suggesting that the expression of some of the genes without known roles in thymocyte development and of novel genes whose expression is found to be altered may also be involved in this process.
Mots-clé
Animals, Antibodies, Monoclonal/immunology, Antibody Specificity, Calcium/metabolism, Cell Differentiation/immunology, Endocytosis/physiology, Gene Expression Profiling, Gene Expression Regulation, Developmental/immunology, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, alpha-beta/immunology, Signal Transduction/immunology, T-Lymphocytes/cytology, T-Lymphocytes/immunology, Transcription, Genetic
Pubmed
Web of science
Création de la notice
13/09/2013 13:46
Dernière modification de la notice
20/08/2019 14:29
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