Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways

Détails

ID Serval
serval:BIB_39D658E9FF8C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways
Périodique
Nature
Auteur⸱e⸱s
Lowin  B., Hahne  M., Mattmann  C., Tschopp  J.
ISSN
0028-0836 (Print)
Statut éditorial
Publié
Date de publication
08/1994
Volume
370
Numéro
6491
Pages
650-2
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 25
Résumé
The recent generation of perforin knock-out mice has demonstrated a crucial role for the pore-forming perforin in cytolytic T-lymphocyte (CTL)-mediated cytolysis. Perforin-deficient mice failed to clear lymphocytic choriomeningitis virus in vivo, yet substantial killing activity still remained in perforin-free CTLs in vitro, indicating the presence of (a) further lytic pathway(s). Fas is an apoptosis-signalling receptor molecule on the surface of a number of different cells. Here we report that both perforin-deficient and Fas-ligand-deficient CTLs show impaired lytic activity on all target cells tested. The killing activity was completely abolished when both pathways were inactivated by using target cells from Fas-receptor-deficient lpr mice and perforin-free CTL effector cells. Fas-ligand-based killing activity was triggered upon T-cell receptor occupancy and was directed to the cognate target cell. Thus, two complementary, specific cytotoxic mechanisms are functional in CTLs, one based on the secretion of lytic proteins and one which depends on cell-surface ligand-receptor interaction.
Mots-clé
Animals Antigens, CD95 Antigens, Surface/*immunology Cell Death Cells, Cultured Cytotoxicity, Immunologic/*immunology H-2 Antigens/immunology Membrane Glycoproteins/*immunology Mice Mice, Inbred C3H Mice, Inbred CBA Mice, Knockout Pore Forming Cytotoxic Proteins Receptors, Antigen, T-Cell/*immunology T-Lymphocytes, Cytotoxic/*immunology Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
24/01/2008 15:17
Dernière modification de la notice
20/08/2019 13:29
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