The stereostructure of knots and catenanes produced by phage lambda integrative recombination: implications for mechanism and DNA structure.

Détails

ID Serval
serval:BIB_394C8ADDE2F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The stereostructure of knots and catenanes produced by phage lambda integrative recombination: implications for mechanism and DNA structure.
Périodique
Cell
Auteur⸱e⸱s
Spengler S.J., Stasiak A., Cozzarelli N.R.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
08/1985
Volume
42
Numéro
1
Pages
325-334
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
We studied the mechanism of recombination by determining the structure of the products of the phage lambda Int system. Electron microscopy of RecA-coated products revealed only knots and catenanes containing a regular right-handed spiral structure. The structure and distribution of products establish that the recombination sites pair by essentially random collision, rather than by tracking. However, the distribution also indicates that the binding of the enzyme must introduce nonrandom components into the reaction and stabilize at least two additional supercoils that become links in the product. Moreover, the regularity of the structures indicates that the strand exchange is accomplished in a very simple way, introducing only a single link into the product. All other links result from the direct conversion of substrate supercoils into knot and catenane links. These supercoils must be in a right-handed, braided form, rather than solenoidally wound as in nucleosomes.
Mots-clé
Bacteriophage lambda/genetics, DNA, Bacterial, DNA, Superhelical, DNA, Viral, Escherichia coli, Lysogeny, Nucleic Acid Conformation, Recombination, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 10:36
Dernière modification de la notice
20/08/2019 13:28
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