Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade.

Détails

ID Serval
serval:BIB_38FC401FF5DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Parallel feedback loops control the basal activity of the HOG MAPK signaling cascade.
Périodique
Integrative Biology
Auteur⸱e⸱s
Sharifian H., Lampert F., Stojanovski K., Regot S., Vaga S., Buser R., Lee S.S., Koeppl H., Posas F., Pelet S., Peter M.
ISSN
1757-9708 (Electronic)
ISSN-L
1757-9694
Statut éditorial
Publié
Date de publication
2015
Volume
7
Numéro
4
Pages
412-422
Langue
anglais
Résumé
Tight regulation of the MAP kinase Hog1 is crucial for survival under changing osmotic conditions. Interestingly, we found that Hog1 phosphorylates multiple upstream components, implying feedback regulation within the signaling cascade. Taking advantage of an unexpected link between glucose availability and Hog1 activity, we used quantitative single cell measurements and computational modeling to unravel feedback regulation operating in addition to the well-known adaptation feedback triggered by glycerol accumulation. Indeed, we found that Hog1 phosphorylates its activating kinase Ssk2 on several sites, and cells expressing a non-phosphorylatable Ssk2 mutant are partially defective for feedback regulation and proper control of basal Hog1 activity. Together, our data suggest that Hog1 activity is controlled by intertwined regulatory mechanisms operating with varying kinetics, which together tune the Hog1 response to balance basal Hog1 activity and its steady-state level after adaptation to high osmolarity.
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/05/2015 12:45
Dernière modification de la notice
20/08/2019 13:28
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