Exploring Extracellular Vesicles Biogenesis in Hypothalamic Cells through a Heavy Isotope Pulse/Trace Proteomic Approach.

Détails

Ressource 1Télécharger: 32466345_BIB_38F5E271C09D.pdf (2876.98 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_38F5E271C09D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Exploring Extracellular Vesicles Biogenesis in Hypothalamic Cells through a Heavy Isotope Pulse/Trace Proteomic Approach.
Périodique
Cells
Auteur⸱e⸱s
Tan C.F., Teo H.S., Park J.E., Dutta B., Tse S.W., Leow M.K., Wahli W., Sze S.K.
ISSN
2073-4409 (Electronic)
ISSN-L
2073-4409
Statut éditorial
Publié
Date de publication
25/05/2020
Peer-reviewed
Oui
Volume
9
Numéro
5
Pages
1320.
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Studies have shown that the process of extracellular vesicles (EVs) secretion and lysosome status are linked. When the lysosome is under stress, the cells would secrete more EVs to maintain cellular homeostasis. However, the process that governs lysosomal activity and EVs secretion remains poorly defined and we postulated that certain proteins essential for EVs biogenesis are constantly synthesized and preferentially sorted to the EVs rather than the lysosome. A pulsed stable isotope labelling of amino acids in cell culture (pSILAC) based quantitative proteomics methodology was employed to study the preferential localization of the newly synthesized proteins into the EVs over lysosome in mHypoA 2/28 hypothalamic cell line. Through proteomic analysis, we found numerous newly synthesized lysosomal enzymes-such as the cathepsin proteins-that preferentially localize into the EVs over the lysosome. Chemical inhibition against cathepsin D promoted EVs secretion and a change in the EVs protein composition and therefore indicates its involvement in EVs biogenesis. In conclusion, we applied a heavy isotope pulse/trace proteomic approach to study EVs biogenesis in hypothalamic cells. The results demonstrated the regulation of EVs secretion by the cathepsin proteins that may serve as a potential therapeutic target for a range of neurological disorder associated with energy homeostasis.
Mots-clé
Animals, Cathepsins/metabolism, Cell Line, Cluster Analysis, Endosomal Sorting Complexes Required for Transport/metabolism, Extracellular Vesicles/metabolism, Extracellular Vesicles/ultrastructure, Gene Ontology, Hypothalamus/cytology, Isotopes/metabolism, Lysosomes/metabolism, Mice, Protein Biosynthesis, Proteome/metabolism, Proteomics/methods, cathepsin, energy homeostasis, extracellular vesicles, extracellular vesicles biogenesis, hypothalamus, pulsed-SILAC
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/06/2020 21:28
Dernière modification de la notice
12/01/2022 7:09
Données d'usage