Kidney gene expression analysis in a rat model of intrauterine growth restriction reveals massive alterations of coagulation genes.

Détails

ID Serval
serval:BIB_38ED9C5D92C1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Kidney gene expression analysis in a rat model of intrauterine growth restriction reveals massive alterations of coagulation genes.
Périodique
Endocrinology
Auteur⸱e⸱s
Buffat C., Boubred F., Mondon F., Chelbi S.T., Feuerstein J.M., Lelièvre-Pégorier M., Vaiman D., Simeoni U.
ISSN
0013-7227 (Print)
ISSN-L
0013-7227
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
148
Numéro
11
Pages
5549-5557
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
In this study, low birth weight was induced in rats by feeding the dams with a low-protein diet during pregnancy. Kidneys from the fetuses at the end of gestation were collected and showed a reduction in overall and relative weight, in parallel with other tissues (heart and liver). This reduction was associated with a reduction in nephrons number. To better understand the molecular basis of this observation, a transcriptome analysis contrasting kidneys from control and protein-deprived rats was performed, using a platform based upon long isothermic oligonucleotides, strengthening the robustness of the results. We could identify over 1800 transcripts modified more than twice (772 induced and 1040 repressed). Genes of either category were automatically classified according to functional criteria, making it possible to bring to light a large cluster of genes involved in coagulation and complement cascades. The promoters of the most induced and most repressed genes were contrasted for their composition in putative transcription factor binding sites, suggesting an overrepresentation of the AP1R binding site, together with the transcription induction of factors actually binding to this site in the set of induced genes. The induction of coagulation cascades in the kidney of low-birth-weight rats provides a putative rationale for explaining thrombo-endothelial disorders also observed in intrauterine growth-restricted human newborns. These alterations in the kidneys have been reported as a probable cause for cardiovascular diseases in the adult.
Mots-clé
Animals, Blood Coagulation Factors/genetics, Complement System Proteins/genetics, Disease Models, Animal, Female, Fetal Growth Retardation/genetics, Fetal Growth Retardation/pathology, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Kidney/embryology, Kidney/metabolism, Oligonucleotide Array Sequence Analysis, Pregnancy, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/02/2015 10:01
Dernière modification de la notice
20/08/2019 14:28
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