Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study

Détails

ID Serval
serval:BIB_38A276F2AF1E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study
Périodique
Journal of Acquired Immune Deficiency Syndromes
Auteur(s)
Burgisser  P., Vernazza  P., Flepp  M., Boni  J., Tomasik  Z., Hummel  U., Pantaleo  G., Schupbach  J.
ISSN
1525-4135 (Print)
Statut éditorial
Publié
Date de publication
02/2000
Volume
23
Numéro
2
Pages
138-44
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Résumé
Five methods for the assessment of plasma viral load (VL) were evaluated in 103 seropositive patients infected with various subtypes of HIV-1. The methods included three RNA-based assays (Amplicor Monitor 1.5, Quantiplex version 2.0, NucliSens), one ultrasensitive reverse transcriptase (PERT) assay and one "boosted" p24 antigen (Ag) enzyme immunoassay (EIA). Subtyping was based on sequencing in env. The sensitivities were, in decreasing order, Amplicor > PERT > p24 Ag > NucliSens > Quantiplex. The low sensitivity of NucliSens was related to the missing of several non-B (A, E, F, G) or recombinant strains, whereas that of Quantiplex did not depend on subtype. In the 1 group O sample and 4 group M samples, only PERT assay or p24Ag EIA produced a positive result. In the quantitative range, correlation was best between Amplicor and Quantiplex (r = 0.8848), fair between Amplicor and NucliSens (r = 0.7064) or PERT assay (r = 0.7266), lowest between Amplicor and p24Ag EIA (r = 0.3989). Amplicor underestimated VL in 1 subtype E sample. Thus, Amplicor performed best in terms of sensitivity (compared with all other assays) and accuracy (compared with NucliSens, PERT assay, and p24Ag) for non-B subtypes in group M samples. PERT assay appears useful for VL assessment in infections by group O or other highly divergent viruses.
Mots-clé
HIV Infections/*blood/virology HIV-1/*classification Humans Immunoenzyme Techniques Polymerase Chain Reaction RNA, Viral/analysis RNA-Directed DNA Polymerase/analysis *Viral Load
Pubmed
Web of science
Création de la notice
25/01/2008 16:14
Dernière modification de la notice
20/08/2019 14:27
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