The early visual system processes different spatial frequencies (SFs) separately. To examine where in the brain the scale-specific information is integrated, we mapped the neural assemblies engaged in interhemispheric coupling with electroencephalographic (EEG) coherence and blood-oxygen-level dependent (BOLD) signal. During similar EEG and functional magnetic resonance imaging (fMRI) experiments, our subjects viewed centrally presented bilateral gratings of different SF (0.25-8.0 cpd), which either obeyed Gestalt grouping rules (iso-oriented, IG) or violated them (orthogonally oriented, OG). The IG stimuli (0.5-4.0 cpd) synchronized EEG at discrete beta frequencies (beta1, beta2) and increased BOLD (0.5 and 2.0 cpd tested) in ventral (around collateral sulcus) and dorsal (parieto-occipital fissure) regions compared with OG. At both SF, the beta1 coherence correlated with the ventral activations, whereas the beta2 coherence correlated with the dorsal ones. Thus distributed neural substrates mediated interhemispheric integration at single SF. The relative impact of the ventral versus dorsal networks was modulated by the SF of the stimulus.