A cell biological view of the siderophore pyochelin iron uptake pathway in Pseudomonas aeruginosa.

Détails

ID Serval
serval:BIB_3840F97E92A7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A cell biological view of the siderophore pyochelin iron uptake pathway in Pseudomonas aeruginosa.
Périodique
Environmental Microbiology
Auteur⸱e⸱s
Cunrath O., Gasser V., Hoegy F., Reimmann C., Guillon L., Schalk I.J.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Statut éditorial
Publié
Date de publication
2015
Volume
17
Numéro
1
Pages
171-185
Langue
anglais
Résumé
Pyochelin (PCH) is a siderophore produced and secreted by Pseudomonas aeruginosa for iron capture. Using (55) Fe uptake and binding assays, we showed that PCH-Fe uptake in P. aeruginosa involves, in addition to the highly studied outer membrane transporter FptA, the inner membrane permease FptX, which recognizes PCH-(55) Fe with an affinity of 0.6 ± 0.2 nM and transports the ferri-siderophore complex from the periplasm into the cytoplasm: fptX deletion inhibited (55) Fe accumulation in the bacterial cytoplasm. Chromosomal replacement was used to generate P. aeruginosa strains producing fluorescent fusions with FptX, PchR (an AraC regulator), PchA (the first enzyme involved in the PCH biosynthesis) and PchE (a non-ribosomic peptide-synthetase involved in a further step). Fluorescence imaging and cellular fractionation showed a uniform repartition of FptX in the inner membrane. PchA and PchE were found in the cytoplasm, associated to the inner membrane all over the bacteria and also concentrated at the bacterial poles. PchE clustering at the bacterial poles was dependent on PchA expression, but on the opposite PchA clustering and membrane association was PchE-independent. PchA and PchE cellular organization suggests the existence of a siderosome for PCH biosynthesis as previously proposed for pyoverdine biosynthesis (another siderophore produced by P. aeruginosa).
Pubmed
Web of science
Création de la notice
08/03/2015 13:02
Dernière modification de la notice
20/08/2019 13:27
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