Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys

Détails

ID Serval
serval:BIB_382120F24394
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys
Périodique
Haematologica
Auteur⸱e⸱s
Drouet M., Mayol J. F., Norol F., Peinnequin A., Zarski J. P., Letoublon C., Herodin F.
ISSN
1592-8721 (Electronic)
ISSN-L
0390-6078
Statut éditorial
Publié
Date de publication
02/2007
Volume
92
Numéro
2
Pages
248-51
Langue
anglais
Notes
Drouet, Michel
Mayol, Jean-Francois
Norol, Francoise
Peinnequin, Andre
Zarski, Jean-Pierre
Letoublon, Christian
Herodin, Francis
eng
Research Support, Non-U.S. Gov't
Italy
2007/02/14
Haematologica. 2007 Feb;92(2):248-51. doi: 10.3324/haematol.10746.
Résumé
The aim of this study was to search for hematopoietic potential in the liver of non-human primates. Lethally irradiated (2 x 5 Gy gamma) macaque monkeys were given autologous hepatic mononuclear cells (HMNC) isolated from a liver lobe by perfusion and digestion with 0.1% collagenase. Two monkeys were given intramedullary injections of HMNC (18.6 x 10(6)/kg, 20.4 x 10(6)/kg) and two others were co-transplanted with HMNC (14.35 x 10(6)/kg, 96.5 x 10(6)/kg) and bone marrow mesenchymal stem cells (0.42 x 10(6)/kg, 1.16 x 10(6)/kg). All monkeys exhibited a transient neutrophil recovery from day 22 for 10 days, but failed to produce platelets and remained transfusion-dependent. In conclusion, adult liver stem cells from a monkey model show a low level of in vivo hematopoietic potential, suggesting ex vivo manipulation will be required before clinical use of such cells.
Mots-clé
Animals, Blood Platelets/metabolism, Bone Marrow Transplantation/*methods, Hematopoietic System, Leukocytes, Mononuclear/cytology, Liver/*cytology, Liver Extracts/metabolism, Macaca fascicularis, Mesenchymal Stem Cells/cytology, Neutrophils/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Stem Cell Transplantation/*methods
Pubmed
Création de la notice
02/05/2024 10:41
Dernière modification de la notice
28/05/2024 7:10
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