Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells

Détails

ID Serval
serval:BIB_37C49F443C03
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells
Périodique
Microbiology
Auteur⸱e⸱s
Guo  A., Lasaro  M. A., Sirard  J. C., Kraehenbuhl  J. P., Schifferli  D. M.
ISSN
1350-0872 (Print)
Statut éditorial
Publié
Date de publication
04/2007
Volume
153
Numéro
Pt 4
Pages
1059-69
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Apr
Résumé
Salmonella enterica serovar Typhimurium can be internalized by immature dendritic cells (DCs). The interacting host and bacterial molecules initiating this process remain uncharacterized. The objective of this study was to investigate whether specific fimbriae are involved in the early step of binding and uptake of Salmonella by DCs. Type 1 fimbriated S. enterica serovar Typhimurium or recombinant Escherichia coli expressing the type 1 fimbriae showed a significantly greater ability to attach to murine bone-marrow-derived DCs than non-fimbriated bacteria. The FimH adhesin was required for efficient interactions with DCs, since fimbriated fimH mutants were impaired in both binding and internalization. Finally, the internalization involved a FimH-dependent process but did not require sipB, a gene essential for Salmonella-mediated invasion of mammalian epithelial cells. Collectively, these data suggest that the bacterial interaction of DCs through the type 1 fimbrial adhesin FimH is sufficient to target S. enterica serovar Typhimurium for cellular uptake.
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 13:26
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